F2R Rabbit pAb - P25116

Core Product Specifications and Parameters

Biological Background: F2R Function and Localization

• Proteinase-activated receptor 1 (PAR1), also known as coagulation factor II receptor, thrombin receptor (TR), is a high-affinity G protein-coupled receptor for activated thrombin.
• Binding of thrombin triggers calcium release from intracellular stores via PTX-insensitive G proteins and phospholipase C activation, leading to InsP3 production. Related references: PMID:1672265 PMID:8136362
• Plays a role in platelet activation and vascular development. Related references: PMID:10079109
• Mediates up-regulation of pro-inflammatory cytokines (MCP-1/CCL2, IL6) in response to coagulation factor Xa (F10) in cardiac fibroblasts and endothelial cells. Related references: PMID:30568593 PMID:34831181
• Subcellular localization: Cell membrane.
• Tissue specificity: Expressed in platelets and vascular endothelial cells.
• Key features: Disulfide bond, glycoprotein, phosphoprotein, involved in blood coagulation and hemostasis.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 42-110) is an extracellular region; consider its suitability for detecting both native and denatured F2R in WB and ELISA. Validate in the relevant sample system.
• Reported cross-reactivity with mouse and rat; verify specificity in rodent tissues if using these species.
• For WB, the predicted protein weight is 47 kDa; use appropriate loading controls and consider potential post-translational modifications that may shift migration.

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