Anti-PADI4 Rabbit Polyclonal Antibody for WB, ELISA - Q9UM07

Core Product Specifications and Parameters

Biological Background: PADI4 Function and Localization

• Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Related references: PMID:15339660 PMID:15345777 PMID:16567635

• Citrullinates histone H1 at Arg-54 (H1R54ci), histone H3 at Arg-2, Arg-8, Arg-17 and/or Arg-26 (H3R2ci, H3R8ci, H3R17ci, H3R26ci), and histone H4 at Arg-3 (H4R3ci). Related references: PMID:15339660 PMID:15345777 PMID:16567635

• Mediates citrullination of histone H1 at Arg-54, leading to H1 displacement, global chromatin decondensation, and promotion of pluripotency and stem cell maintenance. Related references: PMID:15339660 PMID:15345777 PMID:16567635

• Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils through H1R54ci formation. Related references: PMID:18209087

• Involved in the formation of neutrophil extracellular traps (NETs) by mediating citrullination of histone H3, initiating chromatin decondensation and nuclear membrane rupture. Related references: PMID:19153223 PMID:25622091 PMID:28574339

• Citrullination of histone H3 prevents methylation by CARM1 and HRMT1L2/PRMT1, thereby repressing transcription. Related references: PMID:15345777

• Citrullinates EP300/P300 at Arg-2142, which favors its interaction with NCOA2/GRIP1. Related references: PMID:15731352

• Located in the cytoplasm and nucleus, also found in cytoplasmic granules; expressed specifically in eosinophils and neutrophils, but not in peripheral monocytes or lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 350-450 of human PADI4; consider verifying sequence homology for mouse cross-reactivity.
• Predicted molecular weight is 74 kDa; for Western blot, validate band size in human or mouse neutrophil or eosinophil lysates.
• For ELISA, optimize coating conditions and antibody dilution using recombinant PADI4 protein or peptide standard.
• As a polyclonal antibody, it may exhibit lot-to-lot variation; include appropriate positive and negative controls in each assay.
• Cross-reactivity has been reported for human and mouse; if using other species, experimental validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated IVD raw material supply, serving diagnostic manufacturers, labs, and research institutes from concept to clinic. For PADI4-related projects, we provide validated antibody pairs, optimized polyclonal and recombinant antibodies, bulk ancillary reagents, and rare target sourcing. Let us bridge your epigenetics and innate immunity research to diagnostic development with reliable, scalable raw materials.