Anti-PAPD7 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5XG87

Core Product Specifications and Parameters

Biological Background: Terminal nucleotidyltransferase 4A (PAPD7/TENT4A) Function and Localization

• Encodes a terminal nucleotidyltransferase, also known as TENT4A, PAPD7, POLS, TRF4-1, and LAK-1.
• Catalyzes the transfer of ATP and GTP onto RNA 3' poly(A) tails, creating heterogeneous poly(A) tails that stabilize mRNAs by protecting them from active deadenylation. Related references: PMID:23376078, PMID:30026317
• Functions as the catalytic subunit of a TRAMP-like complex, adding short poly(A) tails to nuclear RNAs to target them for exosome-mediated degradation.
• Lacks terminal uridylyltransferase activity and does not participate in replication-dependent histone mRNA degradation.
• Localizes to both the cytoplasm and the nucleus (nucleoplasm).
• Contains ATP- and metal-binding sites; subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 303–542 of human PAPD7; homology across species supports the reported cross-reactivity with human, mouse, and rat.
• For Western blot, a band around 85 kDa is expected; consider using lysates from cell lines or tissues expressing PAPD7 as positive controls.
• For IHC-P, perform antigen retrieval and validate staining specificity in appropriate formalin-fixed, paraffin-embedded tissues.
• As a polyclonal antibody, monitor for potential lot-to-lot variation; titration experiments are recommended for each new application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials, technical services, and consulting. For projects involving PAPD7 and RNA biology, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our team also facilitates sourcing of rare target raw materials, supporting your transition from concept to clinic.