Anti-PRPSAP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60256

Core Product Specifications and Parameters

Biological Background: PRPSAP2 Function and Localization

• PRPSAP2 (Phosphoribosyl pyrophosphate synthase-associated protein 2), also known as PAP41, is encoded by the PRPSAP2 gene. The protein exists in multiple isoforms due to alternative splicing.
• It plays a negative regulatory role in the synthesis of 5-phosphoribose 1-diphosphate (PRPP), a key precursor in nucleotide biosynthesis.
• The protein is ubiquitously expressed across human tissues, as indicated by proteomics identification.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function and interactions.
• PRPSAP2 is classified under keywords: Nucleotide biosynthesis, Alternative splicing, Phosphoprotein, and Acetylation.
• The calculated molecular weight from the UniProt entry is 40,926 Da, consistent with the observed band at approximately 41 kDa in western blotting.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fragment spanning amino acids 211-252 of human PRPSAP2. This region may confer specificity to the intended target; however, cross-reactivity with isoforms or related proteins should be evaluated in the relevant sample system.
• For western blotting (WB), consider testing a range of antibody dilutions (e.g., 1:500–1:2000) and validate the detection of a band near 41 kDa in positive control lysates from human, mouse, or rat sources.
• In immunohistochemistry (IHC-P), antigen retrieval conditions and antibody concentration should be optimized for the tissue type and fixation method used.
• For ELISA, pairing with an appropriate capture antibody may be necessary; the performance should be assessed using recombinant PRPSAP2 protein or cell lysates.
• Since PRPSAP2 is involved in nucleotide biosynthesis, consider using this antibody in studies investigating metabolic pathways or cancer cell proliferation, but always include appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PRPSAP2 polyclonal antibody supports research in nucleotide biosynthesis and metabolic regulation, and is part of our comprehensive portfolio of validated antibodies, bulk ancillary reagents, and rare-target sourcing solutions. Whether you need optimized matched antibody pairs, scalable monoclonal production, or customized raw-material procurement, CamelBio streamlines your development pipeline with a responsive B2B partnership.