Anti-PDCD4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q53EL6

Core Product Specifications and Parameters

Biological Background: PDCD4 Function and Localization

• PDCD4, also known as H731 or neoplastic transformation inhibitor protein, is a tumor suppressor and apoptosis regulator encoded by the gene PDCD4.
• It inhibits translation initiation and cap-dependent translation by hindering the interaction between EIF4A1 and EIF4G and suppressing EIF4A helicase activity.
• PDCD4 modulates JNK kinase activation and down-regulates MAP4K1, affecting MAPK85 and JUN-dependent transcription, which is important in inhibiting cell invasion.
• Subcellular localization includes both the nucleus and cytoplasm.
• It is up-regulated in proliferative cells and highly expressed in epithelial cells of the mammary gland, with reduced expression in lung cancer and colon carcinoma, supporting its role as a tumor suppressor.
• Post-translational modifications include phosphorylation and acetylation; it is a phosphoprotein and ubiquitin-conjugated protein with repetitive sequences.
• Alternative splicing generates multiple isoforms.
• Functionally, it may play a role in apoptosis and binds RNA (by similarity).

Experimental Guidance and Technical Tips

• For Western blot, consider using a reduced sample preparation and validate the band at approximately 52 kDa in lysates from human, mouse, or rat tissues or cell lines.
• The immunogen covers the N-terminal region (aa 1–260); therefore the antibody may detect full-length PDCD4 and potentially some isoforms—confirm specificity with appropriate controls.
• For IF/ICC, fix and permeabilize cells optimally; note that PDCD4 localizes to both nucleus and cytoplasm, so expect signal in both compartments.
• Include a positive control such as a cell line with known PDCD4 expression (e.g., proliferative or epithelial cells) and a negative control (e.g., PDCD4 knockdown) to verify antibody performance.
• ELISA protocols may be developed using recombinant PDCD4 protein as a standard; titrate the antibody to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes by providing one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PDCD4 polyclonal antibody is suitable for cancer, apoptosis, and translational control research. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and procurement.