Anti-PADI4 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UM07

Core Product Specifications and Parameters

Biological Background: PADI4 Function and Localization

• PADI4 catalyzes the citrullination (deimination) of arginine residues on histones H1, H3, and H4, playing a key role in the histone code and regulation of stem cell maintenance. It specifically modifies H1 at Arg-54, H3 at Arg-2, Arg-8, Arg-17, and/or Arg-26, and H4 at Arg-3. Related references: PMID:15339660 PMID:15345777 PMID:16567635
• Citrullination of histone H3 by PADI4 prevents methylation by CARM1 and HRMT1L2/PRMT1, leading to transcriptional repression. Related references: PMID:15345777
• PADI4-mediated citrullination of H1 at Arg-54 (H1R54ci) causes H1 displacement from chromatin, resulting in global chromatin decondensation. This process promotes pluripotency and is critical during the innate immune response in neutrophils. Related references: PMID:15339660 PMID:15345777 PMID:16567635 PMID:18209087
• It is essential for the formation of neutrophil extracellular traps (NETs) by mediating histone H3 citrullination, chromatin decondensation, and nuclear membrane rupture. Related references: PMID:19153223 PMID:25622091 PMID:28574339
• PADI4 also citrullinates EP300/p300 at Arg-2142, which enhances interaction with NCOA2/GRIP1. Related references: PMID:15731352
• Subcellularly, PADI4 localizes to the cytoplasm, nucleus, and cytoplasmic granules.
• Tissue specificity: expressed in eosinophils and neutrophils, but not in peripheral monocytes or lymphocytes.
• Keywords highlight its roles as a calcium- and metal-binding hydrolase, chromatin regulator, and involvement in innate immunity and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 290–663, covering a large C-terminal region. The resulting polyclonal antibody should recognize multiple epitopes, potentially providing robust signal across applications.
• In WB, a band around 74 kDa is expected. Validate with an appropriate positive control, such as neutrophil or stem cell lysates.
• For IHC-P, consider using human, mouse, or rat tissue sections. Strong staining is anticipated in eosinophils and neutrophils; optimize antigen retrieval and antibody dilution.
• In ELISA, use recombinant or purified PADI4 protein to assess reactivity. Titrate antibody and detection reagents to establish a suitable working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering comprehensive support from concept to clinic. This PADI4 antibody supports research into chromatin remodeling, NETosis, and gene regulation. Beyond high-quality monoclonal and polyclonal antibodies, we supply validated antibody pairs, bulk ancillary reagents, and rare target raw materials to streamline your assay development.