Anti-SEMA4A (Semaphorin-4A) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H3S1

Core Product Specifications and Parameters

Biological Background: Semaphorin-4A Function and Localization

• Semaphorin-4A (Sema4A, also known as Semaphorin-B) is a transmembrane protein encoded by the SEMA4A gene, belonging to the semaphorin family of guidance cues.
• It functions as a cell surface receptor for plexins (PLXNB1, PLXNB2, PLXNB3, PLXND1) and plays critical roles in cell-cell signaling.
• Sema4A regulates synapse development: it promotes inhibitory synapse formation via PLXNB1 and excitatory synapse formation via PLXNB2, influencing glutamatergic and GABAergic transmission.
• In the immune system, Sema4A primes antigen-specific T-cells and promotes differentiation of Th1 helper cells, contributing to adaptive immunity.
• It inhibits angiogenesis and promotes axon growth cone collapse and inhibition of axonal extension, acting as a repulsive guidance cue.
• Subcellularly, Sema4A localizes to the cell membrane.
• The protein features an immunoglobulin-like domain, disulfide bonds, and a signal peptide; disease-associated mutations are linked to cone-rod dystrophy and retinitis pigmentosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 320-580 of human SEMA4A, which likely lies within the extracellular domain; this region is well-suited for recognizing the native protein in various applications.
• For Western blotting (WB), SEMA4A is predicted at ~84 kDa; consider using lysates from human, mouse, or rat tissues/cells as positive controls due to cross-reactivity.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be required to access the intracellular epitope if the target is membrane-bound; validate staining with appropriate controls.
• For ELISA, the antibody can be used as capture or detection reagent; optimize coating and blocking conditions based on the assay format.
• As this is a polyclonal antibody, lot-to-lot variability may exist; validation in the intended experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a broad portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For research on semaphorin signaling, neurodevelopment, or immune regulation, we offer this SEMA4A polyclonal antibody along with customized sourcing of rare-target reagents to accelerate your assay development from concept to clinic.