[KO Validated] Anti-PDK1/PDPK1 Rabbit pAb for WB, IHC-P, ELISA - O15530

Core Product Specifications and Parameters

Biological Background: PDK1/PDPK1 Function and Localization

• 3-phosphoinositide-dependent protein kinase 1 (PDK1/PDPK1) is a serine/threonine kinase that functions as a master kinase, phosphorylating and activating numerous AGC family kinases including AKT/PKB, p70 S6 kinase, p90 RSK, PKC, and SGK, thereby regulating diverse cellular processes (PubMed:10226025, PubMed:10480933, PubMed:10995762). Related references: PMID:10226025 PMID:10480933 PMID:10995762
• It plays a critical role in insulin signal transduction by providing the activating phosphorylation to AKT/PKB, which propagates signals regulating cell proliferation, survival, glucose uptake, and amino acid storage (PubMed:10226025, PubMed:12167717, PubMed:9094314). Related references: PMID:10226025 PMID:12167717 PMID:9094314
• PDK1 negatively regulates TGF-beta signaling by modulating SMAD protein interactions and phosphorylation, preventing nuclear translocation of SMAD3 and SMAD4, and influencing SMAD7 localization (PubMed:17327236). Related references: PMID:17327236
• It activates NF-kappa-B pathway via phosphorylation of IKKB (PubMed:16207722) and participates in focal adhesion regulation through its tyrosine-phosphorylated form (PubMed:14585963). Related references: PMID:16207722 PMID:14585963
• Subcellular localization includes cytoplasm, nucleus, cell membrane, and focal adhesions, enabling versatile signaling roles.
• Ubiquitously expressed, with increased Tyr-9 phosphorylation observed in diseased tissues (lung, liver, colon, breast) compared to normal tissues.
• Key post-translational modifications include phosphorylation and acetylation; it belongs to the kinase superfamily with roles in transcription regulation and cell junction dynamics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 409-556, the C-terminal region of human PDK1. Consider verifying antibody specificity in knockout cell lines if possible.
• For Western blotting, expect a band approximately 63 kDa. Validate with positive and negative controls across human, mouse, and rat lysates.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and validate in tissues with known PDK1 expression, such as lung or colon.
• ELISA applications may require optimization of coating and detection conditions; consider using recombinant PDK1 fragments as standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined sourcing bridge for IVD raw materials. For PDK1-related research and assay development, we supply validated antibodies, recombinant proteins, and ancillary reagents to support applications from early discovery to clinical validation.