Anti-NDRG4 Rabbit Polyclonal Antibody for WB - Q9ULP0

Core Product Specifications and Parameters

Biological Background: NDRG4 Function and Localization

• Protein NDRG4, also known as brain development-related molecule 1 (BDM1) and vascular smooth muscle cell-associated protein 8 (SMAP-8), is a member of the N-myc downstream-regulated gene family.
• Alternative splicing generates multiple isoforms; isoform 1 and 2 are brain-specific, while isoform 3 is expressed in both heart and brain.
• Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for spatial learning and resistance to ischemic neuronal cell death (By similarity).
• May enhance growth factor-induced ERK1/ERK2 phosphorylation, including that triggered by PDGF and FGF, and may attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner.
• Localizes predominantly to the cytoplasm and cytosol.
• Expressed predominantly in brain and heart; in the brain, detected in astrocytes; upregulated in glioblastoma multiforme cells.
• Classified as a phosphoprotein with alternative splicing, identified in proteomic analyses.

Experimental Guidance and Technical Tips

• The immunogen targets an internal region (aa 140-220) of human NDRG4; consider that all isoforms retaining this region may be recognized.
• For WB, anticipate a band around 38 kDa; validate detection in your specific sample system, accounting for possible isoform variations.
• For IF/ICC, optimize fixation and permeabilization conditions given the cytoplasmic localization of NDRG4.
• This polyclonal antibody may be suitable for ELISA; perform checkerboard titrations to determine optimal working dilutions.
• Cross-reactivity is predicted with mouse and rat based on sequence homology; experimental validation in these species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers one-stop IVD raw material solutions, supporting diagnostic manufacturers and laboratories with validated antibodies, antigen pairs, and ancillary reagents. This anti-NDRG4 polyclonal antibody addresses research into neurological disorders, ischemic injury, and cancer, complementing our extensive portfolio of bulk and custom-sourced raw materials. From rare target procurement to large-scale supply, CamelBio accelerates your development from concept to clinic.