Anti-PDE1B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q01064

Core Product Specifications and Parameters

Biological Background: PDE1B Function and Localization

• PDE1B is a dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase that hydrolyzes the second messengers cAMP and cGMP, with a higher affinity for cGMP. Related references: PMID:15260978, PMID:8855339, PMID:9419816
• Activation occurs upon binding of calcium/calmodulin, linking calcium influx to cyclic nucleotide degradation.
• Subcellular localization is primarily cytoplasmic and cytosolic.
• The PDE1 family includes multiple isoforms; PDE1B is a member that undergoes alternative splicing (gene: PDE1B/PDES1B).
• Contains conserved metal-binding motifs that coordinate zinc ions, necessary for enzymatic activity.
• Annotated keywords include calmodulin-binding, phosphoprotein, and proteomics identification, reflecting its regulatory nature.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 61 kDa is expected; consider using whole-cell lysates from human, mouse, or rat tissues known to express PDE1B.
• In IHC-P, antigen retrieval may be beneficial; validate the antibody in positive control tissues such as brain or smooth muscle.
• The immunogen (aa 190-536) may detect multiple splice variants if present in the sample.
• For ELISA, the antibody can be used as a capture or detection reagent with recombinant PDE1B protein as a standard.
• Always include appropriate positive and negative controls to confirm specificity in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a reliable one-stop sourcing platform for IVD raw materials, supporting your development from concept to clinic. For studies of cyclic nucleotide signaling and calcium/calmodulin-mediated pathways, we supply validated antibody pairs, optimized polyclonal antibodies like this anti-PDE1B antibody, and bulk ancillary reagents. Our team also assists with sourcing rare targets and custom antibody development to accelerate your diagnostic assay workflows.