Anti-PD-L2/CD273/B7-DC Rabbit Polyclonal Antibody for WB, ELISA - Q9WUL5

Core Product Specifications and Parameters

Biological Background: PD-L2/CD273/B7-DC Function and Localization

• PD-L2, also known as butyrophilin B7-DC, is a member of the B7 family of immune regulatory ligands, containing immunoglobulin-like domains and structural features typical of co-inhibitory molecules.
• PD-L2 plays a critical role in the induction and maintenance of immune tolerance to self. Related references: PMID:11224527 PMID:11283156 PMID:12719480
• It acts as a ligand for the inhibitory receptor PD-1 (PDCD1), suppressing T-cell proliferation by blocking cell cycle progression and cytokine production. Related references: PMID:11224527 PMID:11283156 PMID:12719480
• PD-L2 localizes to the cell membrane as a single-pass type I membrane protein, with an extracellular region containing Ig-like domains, a transmembrane helix, and a short cytoplasmic tail.
• In mice, PD-L2 is expressed in immature and mature dendritic cells, with high expression in placenta and liver, and lower expression in heart, spleen, lymph nodes, and thymus. It is also found in certain tumor cell lines of lymphoid origin.
• PD-L2 is a glycoprotein with predicted disulfide bonds that likely contribute to its structural stability, and it is annotated as part of the adaptive immunity system in the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full mouse PD-L2 sequence, suggesting the antibody should recognize the extracellular domain under both native and denaturing conditions.
• For Western blot, the predicted molecular weight is approximately 28 kDa (calculated 27.8 kDa from UniProt); reducing SDS-PAGE and careful size monitoring near 28 kDa are recommended.
• For ELISA, consider using the recombinant immunogen as a positive control to standardize coating and detection conditions.
• Cross-reactivity with rat PD-L2 is indicated; validation in the relevant rat sample system is advised if used for rat studies.
• Because PD-L2 is expressed in dendritic cells and certain tumor lines, enrichment or selection of appropriate cell types may improve detection sensitivity in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for immune checkpoint targets like PD-L2. Whether your project requires rare-target sourcing or full assay development support, CamelBio bridges the gap from concept to clinic with reliable B2B supply solutions.