Anti-PAPD4 (GLD2/TUT2) Polyclonal Antibody for WB and ELISA - Q6PIY7

Core Product Specifications and Parameters

Biological Background: PAPD4 Function and Localization

• PAPD4 is a cytoplasmic poly(A) RNA polymerase that adds AMP monomers to the 3′-end of specific mRNAs, forming a poly(A) tail, distinct from the canonical nuclear poly(A) polymerase. Related references: PMID:15070731, PMID:31792053
• It does not play a role in replication-dependent histone mRNA degradation. Related references: PMID:18172165
• PAPD4 also mediates monoadenylation of specific miRNAs, which stabilizes and prolongs the activity of some but not all miRNAs. Related references: PMID:23200856, PMID:31792053
• Subcellularly, PAPD4 is localized in both the cytoplasm and nucleus.
• Expression is enriched in the brain, particularly in the cerebellum, hippocampus, and medulla.
• The protein belongs to the terminal nucleotidyltransferase family and requires metal ions such as magnesium and manganese for activity.

Experimental Guidance and Technical Tips

• For Western blot, consider loading 20–50 µg of total protein lysate from cells or tissues expressing PAPD4 (e.g., brain tissues) and validate the signal at the expected ~56 kDa band.
• The immunogen covers the N-terminal region (aa 1–300), so the antibody may recognize both full-length and truncated forms; confirm specificity in your sample system.
• For ELISA, optimize coating and detection conditions using recombinant PAPD4 protein or peptide standards.
• Cross-reactivity is predicted with mouse and rat based on sequence homology, but verify in your model if precise species reactivity is critical.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material partner, CamelBio supports diagnostic developers and researchers with reliable access to high-quality reagents. This anti-PAPD4 polyclonal antibody is well-suited for investigations into cytoplasmic polyadenylation, miRNA regulation, and neurobiology. In addition to primary antibodies, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your workflow from concept to clinic.