Anti-DNA Polymerase Delta Subunit 4 (POLD4/p12) Rabbit Polyclonal Antibody for WB, ELISA - Q9HCU8

Core Product Specifications and Parameters

Biological Background: DNA polymerase delta subunit 4 (POLD4) Function and Localization

• DNA polymerase delta subunit 4 (POLD4), also known as p12 or POLDS, is a component of the tetrameric DNA polymerase delta complex (Pol-delta4). This complex plays a central role in high-fidelity genomic DNA replication and repair. Related references: PMID:16510448, PMID:19074196, PMID:20334433
• Within Pol-delta4, POLD4 increases the rate of DNA synthesis while decreasing fidelity by regulating the polymerase activity and 3' to 5' proofreading exonuclease of the catalytic subunit POLD1. Related references: PMID:16510448, PMID:19074196, PMID:20334433
• POLD4 participates in Okazaki fragment maturation during lagging-strand DNA synthesis, facilitating both the short flap cleavage pathway and a nick translation system. Related references: PMID:24035200
• Under DNA replication stress, POLD4 is required for the repair of broken replication forks via break-induced replication (BIR), a mechanism that can generate segmental genomic duplications of up to 200 kb. Related references: PMID:24310611
• POLD4 is involved in translesion synthesis (TLS) across DNA lesions such as O6-methylguanine and abasic sites, allowing replication to bypass certain types of damage. Related references: PMID:19074196
• In response to DNA damage, POLD4 is targeted for degradation, a process critical for the inhibition of fork progression and promotion of cell survival. This degradation is likely mediated by ubiquitination. Related references: PMID:24022480
• POLD4 is localized to the nucleus, consistent with its essential functions in DNA replication and repair.
• POLD4 is characterized by keywords including alternative splicing, DNA damage, DNA repair, DNA replication, and Ubl conjugation, reflecting its multifaceted role in maintaining genomic stability.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant protein covering amino acids 1–107 of human POLD4, which represents the full-length protein. This design is expected to generate antibodies that recognize the native conformation of POLD4.
• For Western blot (WB) analysis, use a human cell line known to express POLD4; the predicted molecular weight is approximately 12 kDa. Keep in mind that post-translational modifications or protein complex formation might cause slight shifts in migration.
• As a rabbit polyclonal antibody, this reagent likely binds multiple epitopes, providing robust signal even in the presence of minor sequence variations. However, cross-reactivity with orthologs from other species should be empirically determined.
• In ELISA applications, the antibody can be employed in direct, indirect, or sandwich formats. Optimal working dilutions and assay conditions should be optimized by the end user.
• Because POLD4 functions as part of a multi-subunit complex, consider performing co-immunoprecipitation or native gel electrophoresis to study its interactions with other polymerase delta components.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including antibodies such as this anti-POLD4 rabbit polyclonal antibody, to accelerate your DNA replication and repair research and diagnostic assay development. As a trusted one-stop sourcing partner, CamelBio offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for difficult-to-obtain targets. Whether you need consistent lot-to-lot performance or technical consultation, CamelBio is dedicated to supporting your journey from concept to clinical application.