Anti-PADI4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UM07

Core Product Specifications and Parameters

Biological Background: PADI4 Function and Localization

• PADI4 (Protein-arginine deiminase type-4) is a calcium-dependent hydrolase that catalyzes the citrullination (deimination) of arginine residues in target proteins, playing a key role in post-translational modification and chromatin regulation.
• It citrullinates histones: H1 at Arg-54 (H1R54ci), H3 at Arg-2/8/17/26 (H3R2ci, H3R8ci, H3R17ci, H3R26ci), and H4 at Arg-3 (H4R3ci), thereby modulating nucleosome dynamics and gene expression.
  Related references: PMID:15339660 PMID:15345777 PMID:16567635
• In stem cells, PADI4-mediated citrullination of H1R54ci displaces histone H1 from chromatin, inducing global chromatin decondensation and promoting pluripotency maintenance.
  Related references: PMID:15339660 PMID:15345777 PMID:16567635
• During innate immune responses in neutrophils, PADI4 drives profound chromatin decondensation and is essential for the formation of neutrophil extracellular traps (NETs), which trap and eliminate pathogens.
  Related references: PMID:18209087 PMID:19153223 PMID:25622091 PMID:28574339
• Citrullination of histone H3 by PADI4 prevents its methylation by the arginine methyltransferases CARM1 and PRMT1, leading to transcriptional repression.
  Related references: PMID:15345777
• Beyond histones, PADI4 also citrullinates non-histone substrates such as the acetyltransferase EP300 (p300) at Arg-2142, which enhances its interaction with the coactivator NCOA2.
  Related references: PMID:15731352
• PADI4 localizes to the cytoplasm, nucleus, and cytoplasmic granules, consistent with its roles in both cytosolic and nuclear processes.
• Its expression is primarily restricted to eosinophils and neutrophils; it is not detected in peripheral monocytes or lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 290–663, encompassing the C-terminal catalytic domain; the antibody is expected to detect full-length PADI4.
• For Western blotting (WB), the predicted molecular weight is 74 kDa; consider using neutrophil cell lines (e.g., HL-60) or primary granulocytes as positive controls.
• In immunofluorescence (IF/ICC), expect a cytoplasmic, nuclear, and granular staining pattern; permeabilization and fixation protocols may need optimization.
• For ELISA applications, validate the antibody pair with recombinant PADI4 protein or cell lysates to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for one-stop IVD raw-material sourcing, offering everything from validated antibodies and antigens to technical consultancy for diagnostic development. This anti-PADI4 polyclonal antibody supports research into citrullination pathways, NETosis, and autoimmune diseases, and can be complemented by CamelBio’s range of monoclonal antibodies, recombinant proteins, and bulk ancillary reagents tailored for your immunoassay needs.