Anti-REG4 Rabbit Polyclonal Antibody for WB and ELISA - Q9BYZ8

Core Product Specifications and Parameters

Biological Background: REG4 Function and Localization

• Also known as GISP, RELP, REG-IV, Reg IV, this protein is a member of the regenerating islet-derived protein family.
• Functions as a calcium-independent lectin with mannose-binding specificity, capable of maintaining carbohydrate recognition in acidic environments.
• May play a role in inflammatory and metaplastic responses of the gastrointestinal epithelium.
• Highly expressed in the gastrointestinal tract, including duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas, and small intestine; weakly expressed in normal colon and stomach.
• Strongly upregulated in most colorectal tumors compared to normal colon, with preferential expression in mucinous and neuro-endocrine tumors; localized to mucus-secreting cells and enterocyte-like cells.
• Contains a signal peptide and is secreted; post-translational modifications include glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23–158 of human REG4, covering the majority of the mature protein; the antibody may detect endogenous REG4 in western blot (WB) at ~18 kDa.
• For WB, consider optimizing protein loading and antibody dilution, and validate in colorectal cancer cell lines or tissue lysates where REG4 is highly expressed.
• The antibody cross-reacts with mouse and rat REG4, making it suitable for multi-species studies; however, confirm cross-reactivity in your specific experimental system.
• ELISA applications may require pairing with a matched detection antibody; verify performance with recombinant REG4 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by providing a one-stop portfolio that includes validated antibody pairs, optimized polyclonal antibodies like this REG4 pAb, and associated ancillary reagents. Backed by deep expertise in gastrointestinal and cancer biomarker development, we support your research pipeline from target validation to assay manufacture. For REG4-related gastrointestinal disease projects, we offer tailored sourcing for rare targets and bulk supply to meet your production needs.