Anti-PADI2 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y2J8

Core Product Specifications and Parameters

Biological Background: PADI2 Function and Localization

• PADI2, also known as protein-arginine deiminase type-2, PAD-H19, or peptidylarginine deiminase II, is a calcium-dependent hydrolase that catalyzes the deimination (citrullination) of arginine residues on target proteins.
• The enzyme plays a role in post-translational modification, particularly in the conversion of arginine to citrulline, influencing protein structure and function.
• PADI2 is encoded by the PADI2 gene (Gene ID: 11240) and is subject to alternative splicing, generating multiple isoforms.
• Subcellular localization is predominantly cytoplasmic, as annotated in UniProt (Q9Y2J8).
• Tissue expression is prominent in keratinocytes of the epidermis, suggesting a role in skin biology.
• The protein requires calcium and metal ions for enzymatic activity, classifying it as a metal-binding hydrolase.
• Structural studies (3D-structure keyword) provide insights into its domain organization and catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-200 of human PADI2; consider performing titration experiments to determine the optimal working dilution for WB and ELISA applications.
• Given the cytoplasmic localization of PADI2, prepare whole-cell lysates or cytoplasmic extracts for western blot analysis.
• Validate antibody specificity using appropriate controls, such as PADI2-overexpressing lysates or blocking peptides, in the relevant human sample system.
• For ELISA, consider direct or sandwich formats, ensuring adequate coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement solution for IVD raw materials, including this PADI2 rabbit polyclonal antibody targeting protein-arginine deiminase type-2. As a one-stop sourcing partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic and research workflows from concept to clinic.