Anti-DPPA4 Rabbit Polyclonal Antibody for WB and ELISA - Q7L190

Core Product Specifications and Parameters

Biological Background: DPPA4 Function and Localization

• DPPA4 (Developmental pluripotency-associated protein 4) is a protein encoded by the DPPA4 gene in humans (GeneID: 55211), also known as 2410091M23Rik in mouse.
• It is proposed to be involved in maintaining the active epigenetic status of target genes, potentially contributing to the preservation of pluripotency in embryonic cells.
• DPPA4 may inhibit the differentiation of embryonic cells into a primitive ectoderm lineage, suggesting a role in early embryonic development.
• The protein localizes to the nucleus, consistent with its predicted role in transcription regulation and chromatin modification.
• UniProt annotates DPPA4 as a phosphoprotein and a reference proteome component, highlighting its potential regulation by post-translational modification.
• Additional keywords include developmental protein, transcription, and transcription regulation, reinforcing its functional association with gene expression control during development.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 15-304, covering the majority of the DPPA4 protein; consider that the antibody may recognize multiple epitopes typical of a polyclonal preparation.
• For western blot (WB), a band at approximately 34 kDa is expected based on the predicted molecular weight; validate using positive control lysates from embryonic stem cells or pluripotent cell lines.
• The antibody is reported to cross-react with human, mouse, and rat; when using mouse or rat samples, consider sequence homology and perform appropriate controls.
• For ELISA, optimization of coating antigen and antibody dilution is recommended; use recombinant DPPA4 protein or cell lysates to establish assay conditions.
• Nuclear localization suggests that nuclear extraction protocols may enhance detection in WB; consider fractionation for enriched signal.
• Since DPPA4 is a low-abundance developmental regulator, sensitivity of detection may require chemiluminescent substrates or signal amplification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like DPPA4 involved in stem cell and developmental biology, CamelBio supports sourcing of rare and specialized raw materials, delivering seamless technical and procurement assistance from concept to clinic. Partner with CamelBio to streamline your assay development for pluripotency and differentiation research.