Anti-PDHA1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P08559

Core Product Specifications and Parameters

Biological Background: PDHA1 Function and Localization

• PDHA1 encodes the somatic form of the pyruvate dehydrogenase E1 component subunit alpha, located in the mitochondrial matrix.
• The E1 subunit, together with PDHB, catalyzes the thiamine pyrophosphate-dependent decarboxylation of pyruvate and the subsequent reductive acetylation of the lipoyl moieties of the E2 component. Related references: PMID:17474719, PMID:19081061, PMID:7782287
• This reaction is the rate-limiting step of the pyruvate dehydrogenase complex, which links glycolysis to the TCA cycle by producing acetyl-CoA.
• PDHA1 is ubiquitously expressed across tissues, reflecting its central metabolic role.
• The protein is subject to regulatory phosphorylation and acetylation, and mutations are associated with primary mitochondrial disease such as Leigh syndrome.

Experimental Guidance and Technical Tips

• The immunogen spans a central region (aa 30–390) of human PDHA1, which is highly conserved; cross‑reactivity with mouse and rat is expected based on sequence homology.
• For Western blot analysis, detect a band at approximately 43 kDa under reducing conditions; verify specificity using appropriate positive and negative controls.
• For immunohistochemical (IHC-P) staining, antigen retrieval may be necessary; consider using liver or muscle sections as positive controls.
• This polyclonal antibody is compatible with immunoprecipitation (IP) and ELISA; it is recommended to titer the antibody for each assay system to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-PDHA1 reagent, and a broad portfolio of ancillary reagents. Whether you need bulk supplies, rare target sourcing, or technical consultation from concept to clinic, CamelBio supports your diagnostic and research workflows with reliable, high-quality products.