Anti-PADI1 Rabbit Polyclonal Antibody for WB and ELISA - Q9ULC6

Core Product Specifications and Parameters

Biological Background: PADI1 Function and Localization

• PADI1 (Protein-arginine deiminase type-1), also known as peptidylarginine deiminase I, is encoded by the PADI1 gene (aliases PDI, PAD1, PDI1, HPAD10).
• Catalyzes the calcium-dependent deimination (citrullination) of arginine residues in proteins, converting positively charged arginine to neutral citrulline.
• PADI1 is a member of the protein-arginine deiminase family, which includes several isoforms with tissue-specific expression.
• Subcellularly localized in the cytoplasm, where it modifies target proteins.
• Highly expressed in epidermal keratinocytes, with detectable expression also in prostate, testis, placenta, spleen, and thymus.
• Keywords: Hydrolase, Calcium, Metal-binding, emphasizing its enzymatic and calcium-binding properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-277) of human PADI1, providing targeted specificity.
• For Western blotting, consider a standard protocol with a primary antibody dilution suitable for detecting endogenous PADI1; a band of approximately 75 kDa is expected based on the calculated molecular weight.
• For ELISA, the antibody can be used for capture or detection of recombinant or native PADI1; validate optimal coating and detection conditions in your system.
• Cross-reactivity with mouse and rat has been reported, making this antibody suitable for studies in these species; however, verify performance in the specific tissue or cell line of interest.
• As a polyclonal antibody, lot-to-lot variability may exist; it is advisable to titrate the antibody for each new batch and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This anti-PADI1 antibody supports research into protein citrullination and its role in epidermal biology and disease. Contact CamelBio for bulk sourcing, customized antibody services, and technical support to accelerate your diagnostic development.