Anti-OTUB2 Rabbit Polyclonal Antibody for WB, ELISA - Q96DC9

Core Product Specifications and Parameters

Biological Background: OTUB2 Function and Localization

• OTUB2 is a ubiquitin thioesterase belonging to the OTU (ovarian tumor) domain-containing deubiquitinase family, also known as Otubain-2.
• It functions as a hydrolase that removes conjugated ubiquitin from proteins, playing a regulatory role in protein turnover by preventing degradation.
• OTUB2 mediates deubiquitination of 'Lys-11'-, 'Lys-48'-, and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked chains.
• The protein is widely expressed, with higher levels observed in the brain (tissue specificity from UniProt).
• It is annotated with keywords including Hydrolase, Protease, Thiol protease, and Ubl conjugation pathway, indicating its enzymatic role in ubiquitin-like modification.
• Alternative splicing of the OTUB2 gene generates multiple transcript variants (from Keywords: Alternative splicing).

Experimental Guidance and Technical Tips

• For Western blot, validate the appropriate primary antibody dilution in your sample system, targeting the ~27 kDa band.
• The immunogen (N-terminal amino acids 1–73) recognizes full-length OTUB2, but consider potential cross-reactivity with splice variants or isoforms.
• In ELISA applications, confirm linearity and sensitivity using recombinant OTUB2 protein as a standard; both direct and sandwich formats can be explored.
• As a polyclonal antibody, include positive and negative controls to account for possible lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, from concept to clinic. This anti-OTUB2 rabbit polyclonal antibody serves ubiquitin-proteasome and deubiquitinase research needs. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials.