Anti-BTNL2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UIR0

Core Product Specifications and Parameters

Biological Background: BTNL2 Function and Localization

• Butyrophilin-like protein 2 (BTNL2), also known as SS2, BTN7, BTL-II, or HSBLMHC1, is a transmembrane protein with immunoglobulin-like domains, encoded by the BTNL2 gene.
• BTNL2 functions as a negative regulator of T-cell proliferation, likely contributing to immune tolerance and preventing excessive immune activation.
• Structural similarity to the B7 family of co-stimulatory molecules suggests a role in modulating T-cell responses through receptor-ligand interactions.
• Subcellularly, BTNL2 localizes to the membrane via a signal-anchor sequence, with a single transmembrane helix anchoring it in the lipid bilayer.
• BTNL2 is broadly expressed in tissues such as brain, heart, kidney, liver, pancreas, ovary, leukocyte, small intestine, testis, and thymus.
• The protein undergoes glycosylation and contains disulfide bonds, which may stabilize its extracellular immunoglobulin domains and influence ligand binding.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 246-455 of human BTNL2, which corresponds to its extracellular domain; consider this when selecting detection strategies.
• For Western blot, a band around 50 kDa is expected; validate with positive and negative controls from relevant tissues or cell lines.
• In immunohistochemistry (paraffin-embedded), antigen retrieval conditions may need optimization.
• For ELISA, the antibody can be used as both capture and detection reagent; pairing with another clone may improve sensitivity.
• Cross-reactivity with mouse and rat suggests conserved epitopes; suitability should be verified in the intended model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, technical services, and consulting. This BTNL2 antibody supports immunology and immune-oncology research, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare-target raw material sourcing or custom development needs, CamelBio offers tailored procurement support.