Anti-AMOTL2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y2J4

Core Product Specifications and Parameters

Biological Background: AMOTL2 Function and Localization

• AMOTL2 (Angiomotin-like protein 2), also known as Leman coiled-coil protein (LCCP), is a multi-domain adaptor protein belonging to the angiomotin family.
• AMOTL2 regulates actin cytoskeleton architecture, anchoring radial actin fibers to CDH1-based adherens junctions and enabling cellular response to contractile forces, thereby contributing to epithelial sheet integrity (PubMed:28842668). Related references: PMID:28842668
• It inhibits Wnt/β-catenin signaling by promoting CTNNB1 sequestration in recycling endosomes, and activates the Hippo pathway via WWP1-mediated ubiquitination, which recruits LATS2 to phosphorylate YAP1, leading to its cytoplasmic retention and inhibition of YAP1-driven transcription (PubMed:34404733, PubMed:17293535, PubMed:21205866, PubMed:26598551). Related references: PMID:34404733 PMID:17293535 PMID:21205866 PMID:26598551
• AMOTL2 also sequesters the transcriptional coactivator WWTR1/TAZ in the cytoplasm and at tight junctions, further suppressing YAP/TAZ target gene expression (PubMed:23911299). Related references: PMID:23911299
• At the subcellular level, AMOTL2 localizes to recycling endosomes, the cytoplasm, cell projections, podosomes, and cell junctions.
• It regulates podosome cortex and core size and composition at myofibril neuromuscular junctions (PubMed:23525008). Related references: PMID:23525008
• AMOTL2 selectively promotes FGF-induced MAPK activation through SRC (PubMed:17293535). Related references: PMID:17293535

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 638–837) of human AMOTL2; the antibody may detect full-length and potential isoforms or degradation products.
• For WB, expect a band at ~86 kDa; consider using endothelial (e.g., HUVEC) or epithelial cell lysates as positive controls. Both human and mouse samples are reported cross-reactive.
• For IHC-P and IF/ICC, staining may be observed at cell–cell junctions, cytoplasm, and podosome-like structures; optimize fixation and permeabilization conditions for your specific sample.
• ELISA may be performed with recombinant AMOTL2 protein or cell lysates; validate typical use as a coating or detection antibody.
• As a polyclonal antibody, lot-specific validation of working dilutions and performance in your intended application is recommended.

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