Anti-OTX2 Rabbit Polyclonal Antibody for WB and ELISA - P32243

Core Product Specifications and Parameters

Biological Background: OTX2 Function and Localization

• Homeobox protein OTX2, also known as orthodenticle homolog 2, is a member of the paired-like homeodomain transcription factor family.
• OTX2 plays essential roles in the embryonic development of the forebrain, midbrain, and sensory structures such as the eyes and ears.
• The protein binds specifically to the bicoid/BCD target sequence (BTS: 5'-TCTAATCCC-3') to regulate downstream gene expression.
• Alternative splicing generates multiple OTX2 isoforms, contributing to functional diversity.
• OTX2 localizes primarily to the nucleus, consistent with its role as a DNA-binding transcriptional regulator.
• Mutations in OTX2 are associated with human developmental disorders, including microphthalmia and combined pituitary hormone deficiency.
• UniProt lists OTX2 under keywords including Alternative splicing, Developmental protein, Disease variant, DNA-binding, Homeobox, and Nucleus, reflecting its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 140-280 of human OTX2 (NP_068374.1), targeting a region that may confer specificity for certain OTX2 isoforms.
• For western blotting (WB), a band around 32 kDa is expected in human samples; consider using nuclear extracts to enrich the target protein.
• This antibody shows cross-reactivity with mouse OTX2, as indicated; validate in mouse tissues relevant to brain or eye development studies.
• In ELISA applications, optimize coating conditions and antibody concentrations for best signal-to-noise ratio.
• Always include appropriate positive and negative controls, such as OTX2-overexpressing lysates and knockout cell lines, if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing bridge for IVD raw materials. For OTX2-related research in developmental biology, neuroscience, and rare disease areas, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support rare target sourcing. Leverage our integrated technical services to streamline your development from concept to clinic.