Anti-POU2F2 (OCT2) Rabbit Polyclonal Antibody for WB and ELISA - P09086

Core Product Specifications and Parameters

Biological Background: POU2F2 Function and Localization

• POU2F2 (POU domain, class 2, transcription factor 2), also known as Oct-2 or OTF-2, is a member of the POU-homeodomain family of transcription factors.

• Specifically binds the octamer motif (5'-ATTTGCAT-3') in the regulatory regions of its target genes. Related references: PMID:2904654, PMID:7859290

• Regulates transcription in a variety of tissues, notably activating immunoglobulin gene expression in B cells. Related references: PMID:2901913, PMID:2904654

• Modulates the transactivation potential of nuclear receptors NR3C1, AR, and PGR. Related references: PMID:10480874

• Regulates IL6 expression in B cells in cooperation with the coactivator POU2AF1 (By similarity).

• Activates the U2 small nuclear RNA (snRNA) promoter.

• Localizes to both the cytoplasm and nucleus, with its nuclear import essential for transcriptional activity.

• Isoform 3 is B-cell specific, while isoform 5 is detected in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in BW5147 T cells.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 400-479) lies within the C-terminal region of POU2F2. Given the polyclonal nature of this antibody, it may recognize multiple epitopes and could detect various isoforms.
• For Western blot, consider using B-cell lines (e.g., Raji, Daudi) or lymphoid tissues as positive controls, as POU2F2 is highly expressed in B cells.
• Validate the antibody in your specific experimental system, as cross-reactivity with mouse and rat has been reported, making it suitable for multi-species studies.
• For ELISA, optimize coating conditions using recombinant protein or cell lysates, and include appropriate negative controls to ensure specificity.
• The reported molecular weight is 51 kDa; use a suitable protein ladder and adjust gel percentage accordingly to achieve clear separation.

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CamelBio stands as a dedicated one-stop partner for diagnostic manufacturers and research labs, streamlining your access to high-quality IVD raw materials from discovery to commercialization. Whether you require validated antibody pairs, optimized polyclonal antibodies like this anti-POU2F2 reagent, bulk ancillary reagents, or specialized sourcing for rare targets in transcription factor signaling and B-cell biology, our comprehensive supply network ensures reliable delivery. We focus on bridging your procurement needs with consistent, scalable solutions—let us support your next breakthrough with efficiency and expertise.