Anti-OSBPL2 Rabbit Polyclonal Antibody for WB, ELISA - Q9H1P3

Core Product Specifications and Parameters

Biological Background: OSBPL2 Function and Localization

• OSBPL2 (Oxysterol-binding protein-related protein 2), also known as ORP2, is an intracellular lipid transport protein that binds sterols and phospholipids, mediating lipid transport between intracellular compartments. Related references: PMID:30581148, PMID:32914503, PMID:34124795
• It increases plasma membrane cholesterol levels and decreases phosphatidylinositol-4,5-bisphosphate (PIP2) levels, controlling LDL-cholesterol plasma membrane delivery, focal adhesion kinase (FAK) activation, and PI(4,5)P2 generation, thereby enhancing cell adhesion. Related references: PMID:30581148, PMID:32914503, PMID:34124795
• Exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate; also binds cholesterol, dehydroergosterol, 22(R)-hydroxycholesterol, and 25-hydroxycholesterol in vitro. Related references: PMID:11279184, PMID:17428193, PMID:19224871, PMID:30581148
• In conjunction with PLCB3, OSBPL2 may contribute to the control of keratinocyte proliferation and differentiation, likely through regulation of the ERK pathway and cell cycle. Related references: PMID:38701954
• Involved in angiogenic signaling pathways such as VEGFR2 in endothelial cells. Related references: PMID:32914503
• Localizes to the cytoplasm, cytosol, lipid droplets, cell membrane, and stereocilia (cell projections).
• Widely expressed across tissues; associated with deafness (DFNA67) and ichthyosis. Keywords include alternative splicing, lipid transport, lipid-binding, and non-syndromic deafness.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment covering amino acids 291-480 of human OSBPL2 (NP_653081.1), which may generate antibodies recognizing multiple epitopes within this region. Consider epitope mapping if specific domain recognition is required.
• For Western blot (WB), a band around 55 kDa is expected; however, validate the observed band size in your specific sample system, as post-translational modifications or isoforms may alter migration.
• ELISA can be used for titer determination or screening; ensure proper coating and detection conditions for the recombinant immunogen or native protein.
• Given the polyclonal nature, cross-reactivity with other OSBP-related proteins cannot be excluded; perform appropriate controls for your target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, supporting projects from concept to clinic. For studies on lipid metabolism, deafness, or cell adhesion, this anti-OSBPL2 antibody is available along with validated antibody pairs, bulk ancillary reagents, and sourcing of rare targets. Our one-stop platform streamlines procurement so you can focus on advancing your diagnostic development.