Anti-ApoB Rabbit Polyclonal Antibody (WB, IF/ICC, ELISA) - P04114

Core Product Specifications and Parameters

Biological Background: Apolipoprotein B-100 Function and Localization

• Apolipoprotein B (ApoB) is a major protein constituent of chylomicrons (apoB-48), LDL (apoB-100), and VLDL (apoB-100). ApoB-48 and apoB-100 arise from the same gene through RNA editing.
• ApoB-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor, playing a critical role in cholesterol homeostasis.
• Subcellularly, ApoB-100 localizes to the cytoplasm, is secreted, and associates with lipid droplets, reflecting its role in lipoprotein assembly and secretion.
• Post-translational modifications include acetylation, disulfide bond formation, glycosylation, palmitoylation, and phosphorylation, which may regulate its function and stability.
• ApoB-100 contains heparin-binding domains and is implicated in atherosclerosis, making it a key target in cardiovascular disease research.

Experimental Guidance and Technical Tips

• For Western blot (WB): Due to the large molecular weight (~516 kDa), use low-percentage polyacrylamide gels (e.g., 4–6%) and extended transfer times with reduced methanol in the transfer buffer to ensure efficient transfer.
• For immunofluorescence/immunocytochemistry (IF/ICC): Fixation and permeabilization conditions should be optimized, as ApoB is both cytoplasmic and secreted; a combination of Triton X-100 permeabilization with paraformaldehyde fixation may be effective.
• For ELISA: The recombinant immunogen (amino acids 28–330) can serve as a useful positive control; consider testing a range of antibody dilutions for optimal signal-to-noise ratio.
• Validate the antibody’s cross-reactivity with mouse and rat samples using appropriate tissue or cell lysates, given the high sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-ApoB polyclonal antibody is ideal for studying lipid metabolism and cardiovascular disease biomarkers. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions for rare targets—ensuring reliable supply chains for your diagnostic development.