Anti-POU2F1/OCT1 Rabbit Polyclonal Antibody for WB, ELISA - P14859

Core Product Specifications and Parameters

Biological Background: POU2F1/OCT1 Function and Localization

• POU2F1, also known as Oct-1, OTF-1, and NF-A1, is a ubiquitously expressed transcription factor belonging to the POU domain class 2 family. Alternative splicing generates multiple isoforms, including a lymphocyte-specific isoform 2.
• It binds specifically to the octamer motif (5'-ATTTGCAT-3') and functions as a transcriptional activator for promoters of small nuclear RNAs (snRNA), histone H2B, and immunoglobulin genes.
• POU2F1 modulates transcriptional transactivation by nuclear hormone receptors such as NR3C1 (glucocorticoid receptor), AR (androgen receptor), and PGR (progesterone receptor), integrating diverse signaling pathways.
• During herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator VP16 and host factor HCFC1, enabling transcription of viral immediate early genes, highlighting its role in host-virus interactions.
• The protein localizes exclusively to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Key structural features include a POU-specific domain and a POU homeodomain that mediate DNA binding, and the protein is subject to phosphorylation, which may regulate its activity and interactions.
• With a molecular weight of approximately 76 kDa, POU2F1 is widely detected across tissues, and its function is critical for both basal and inducible gene expression.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein fragment (aa 15-290) of human POU2F1/OCT1, encompassing the N-terminal region upstream of the POU domain. Verify reactivity in your target species and experimental system.
• For Western blotting, a band around 76 kDa is expected; however, alternative splicing or post-translational modifications may produce additional bands. Optimize loading and blocking conditions.
• ELISA applications may require optimization of coating antigen and antibody concentration; consider using the immunogen or a peptide for direct binding assays.
• Cross-reactivity with rat has been reported, but performance in other species, including mouse, should be tested if needed.
• Polyclonal antibodies may exhibit lot-to-lot variability; validate each lot in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering a broad portfolio of antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. Our POU2F1/OCT1 antibody is part of our transcription factor collection, suitable for researchers and manufacturers developing assays in gene regulation, chromatin biology, and virology. We provide optimized monoclonal and polyclonal antibodies, validated pairs, and customized sourcing for rare targets, ensuring reliable supply for your IVD needs.