Anti-OTUB1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96FW1

Core Product Specifications and Parameters

Biological Background: OTUB1 Function and Localization

• OTUB1 is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-48'-linked polyubiquitin chains, playing a critical role in protein turnover and degradation (PubMed:12401499, 12704427, 14661020). Related references: PMID:12401499 PMID:12704427 PMID:14661020
• The gene encodes two isoforms via alternative splicing: isoform 1 (ubiquitous) destabilizes RNF128 to prevent T-cell anergy, while isoform 2 (lymphoid‑specific) stabilizes RNF128 and promotes anergy (PubMed:14661020). Related references: PMID:14661020
• Besides its catalytic activity, OTUB1 acts non‑catalytically in DNA damage repair by binding free ubiquitin and inhibiting UBE2N/UBC13, thereby limiting RNF168‑mediated 'Lys‑63'‑linked histone ubiquitination (PubMed:20725033, 22325355). Related references: PMID:20725033 PMID:22325355
• OTUB1 deubiquitinates estrogen receptor alpha (ESR1) and can remove NEDD8 conjugates, albeit with lower efficiency than 'Lys‑48'‑linked ubiquitin (PubMed:19383985, 18954305, 23827681). Related references: PMID:19383985 PMID:18954305 PMID:23827681
• Phosphorylation at Tyr‑26 regulates mTORC1 activity by mediating non‑catalytic deubiquitination of RPTOR, while amino acid‑dependent deubiquitination of DEPTOR can inhibit mTORC1 and mTORC2 (PubMed:29382726, 35927303). Related references: PMID:29382726 PMID:35927303
• Subcellular localization is predominantly cytoplasmic.
• Isoform 1 is expressed ubiquitously, whereas isoform 2 is restricted to lymphoid tissues (tonsils, lymph nodes, spleen) and peripheral blood mononuclear cells.
• OTUB1 belongs to the OTU domain family of thiol proteases and is classified as a hydrolase; it participates in immunity, DNA repair, and ubiquitin conjugation pathways.

Experimental Guidance and Technical Tips

• The immunogen covers the N‑terminal region (aa 1‑220) of human OTUB1, which encompasses the OTU domain; observed cross‑reactivity with mouse and rat suggests conservation of this region.
• For western blot, anticipate a band near 31 kDa; use appropriate positive controls (e.g., HeLa or Jurkat lysates) and consider siRNA knockdown to confirm specificity.
• In immunofluorescence/ICC, perform standard fixation and permeabilization; expect cytoplasmic staining patterns.
• For ELISA applications, optimize coating antigen concentration and antibody dilutions; pairing with a detection antibody can support sandwich assay development.
• Although the antibody has been shown to react with human, mouse, and rat samples, always verify performance in your specific experimental system.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this OTUB1 polyclonal antibody for deubiquitinase research and assay development. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance with rare target sourcing, our one‑stop platform supports diagnostic manufacturers from concept to clinic. Contact us to explore custom solutions for ubiquitin pathway targets.