Anti-SLCO2B1 Rabbit Polyclonal Antibody for WB/ELISA - O94956

Core Product Specifications and Parameters

Biological Background: SLCO2B1 Function and Localization

• SLCO2B1 (OATP2B1/OATP-B) is a multi-specific organic anion transporter that mediates Na+-independent uptake of various substrates, including steroid sulfate conjugates (estrone-3-sulfate, DHEA-S, pregnenolone sulfate), prostaglandins (PGD2, PGE1, PGE2), thyroxine, leukotriene C4, and thromboxane B2.
  Related references: PMID:10873595, PMID:11159893, PMID:11932330, PMID:12409283, PMID:12724351, PMID:28408210, PMID:16908597, PMID:25132355, PMID:14610227, PMID:19129463, PMID:29871943
• It plays critical roles in hepatic clearance of organic anions, placental transfer of fetal-derived sulfated steroids, intestinal absorption of xenobiotics, and transport of neurosteroids across the blood-brain barrier.
  Related references: PMID:10873595, PMID:11159893, PMID:11932330, PMID:12409283, PMID:12724351, PMID:28408210, PMID:16908597, PMID:25132355
• Acts as a heme transporter, promoting cellular iron availability via heme oxygenase, and mediates uptake of coproporphyrin III, suggesting a role in heme/porphyrin homeostasis.
  Related references: PMID:35714613, PMID:26383540
• Transport mechanism involves anion exchange, likely using bicarbonate or glutamate as counteranions, and is stimulated by acidic pH; an inwardly directed proton gradient may drive estrone-3-sulfate uptake.
  Related references: PMID:19129463, PMID:20507927, PMID:26277985, PMID:14610227, PMID:22201122
• Subcellularly, the protein localizes to the cell membrane, with tissue-specific polarization: basal membrane in hepatocytes and Sertoli cells, basolateral in syncytiotrophoblast, and apical in enterocytes.
• Highly expressed in liver sinusoidal membranes, kidney, placental trophoblasts, small intestine, blood-brain barrier endothelial cells, retina, skeletal muscle, and testis; also detected at lower levels in pancreas, lung, heart, colon, ovary, and spleen.
  Related references: PMID:10873595, PMID:11159893, PMID:11932330, PMID:12409283, PMID:12724351, PMID:23531488, PMID:25132355, PMID:35307651
• Post-translational modifications include disulfide bond formation, N-glycosylation, and phosphorylation; it is a phosphorylated glycoprotein with multiple transmembrane helices.
• Alternative splicing generates multiple isoforms with distinct tissue distributions: isoform 2 predominant in brain, while isoform 1 predominates in liver.

Experimental Guidance and Technical Tips

• The immunogen (aa 130-210) corresponds to a predicted intracellular loop region, suitable for detecting total SLCO2B1 in denaturing (WB) and native (ELISA) conditions; expect a band at ~77 kDa, with possible higher molecular weight bands due to glycosylation.
• For WB, consider using membrane protein extraction protocols and loading appropriate positive controls such as human liver, placenta, or intestine lysates; verify specificity in siRNA knockdown samples if available.
• In ELISA applications, coating with recombinant SLCO2B1 protein or the immunogen peptide can help assess antibody titer; note that the antibody may recognize mouse SLCO2B1 as indicated by cross-reactivity, but validate in your specific mouse tissue.
• Optimize blocking and antibody dilution to minimize background, especially in tissues with high endogenous peroxidase activity (e.g., liver).
• When studying transport function, remember that SLCO2B1 activity is pH-sensitive; in vitro functional assays may require an acidic buffer (pH 5–6) to mimic the microenvironment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing experience for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-SLCO2B1 polyclonal antibody is part of our expanding portfolio of transporter-targeting reagents, ideal for drug transport studies, hepatic clearance investigations, and placental barrier research. Beyond antibodies, we provide validated antigen pairs, optimized monoclonal options, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development and biomedicine projects.