Anti-OXT Rabbit Polyclonal Antibody for WB, ELISA - P01178

Core Product Specifications and Parameters

Biological Background: OXT Function and Localization

• Precursor protein: OXT encodes the oxytocin-neurophysin 1 proprotein, a precursor that is cleaved to yield the nonapeptide hormone oxytocin and its carrier protein neurophysin 1.
• Social behavior hormone: Oxytocin is a neurohypophyseal hormone that plays a central role in social behaviors such as maternal care, social cognition, and affiliative behaviors. Related references: PMID:15931222 PMID:20538951 PMID:21220339
• Neural modulation: Oxytocin binds to oxytocin receptor (OXTR) and triggers signaling pathways that modulate neural circuits by altering ion channel activity and synaptic transmission, thereby influencing complex behaviors like empathy, trust, and generosity. Related references: PMID:18174156 PMID:35241813 PMID:35851571
• Peripheral functions: Peripherally secreted oxytocin drives uterine smooth muscle contractions during parturition and triggers milk ejection in lactation. Related references: PMID:6278592
• Bone formation: Oxytocin promotes bone mass by stimulating osteoblast differentiation and bone formation (by similarity).
• Carrier protein: Neurophysin 1 binds oxytocin and transports it along axons from hypothalamic neurons to the posterior pituitary, protecting the hormone from proteolytic degradation.
• Subcellular localization: The proprotein localizes to secretory vesicles; upon stimulation, both oxytocin and neurophysin 1 are secreted.
• Post-translational modifications: Mature oxytocin requires C-terminal amidation and an intramolecular disulfide bond between cysteine residues 1 and 6 for full biological activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length proprotein (aa 1-125); the antibody may detect the ~13 kDa precursor and the ~10 kDa neurophysin 1 fragment in reducing Western blot. Validate band identity in appropriate tissue lysates (e.g., hypothalamus, pituitary).
• Cross-reactivity with mouse and rat orthologs makes this antibody suitable for rodent models; confirm species-specific detection in your system.
• For ELISA, consider using the antibody as capture or detection reagent in a sandwich format; pairing with a monoclonal antibody may improve specificity. Optimal working concentrations must be determined experimentally.
• As a polyclonal antibody, lot-to-lot variability can occur; include positive and negative controls when testing a new lot.
• Shipping on ice bags preserves antibody integrity; upon receipt, aliquot and store at -20°C or as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for sourcing high-quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. Whether you are developing oxytocin-related diagnostic assays or researching social behavior, CamelBio’s integrated supply chain and technical expertise can accelerate your project from concept to clinic. Explore our portfolio of optimized antibodies and rare-target reagents tailored for diagnostic manufacturers and research laboratories.