Anti-TUBB8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q3ZCM7

Core Product Specifications and Parameters

Biological Background: Tubulin beta-8 chain Function and Localization

• TUBB8 encodes Tubulin beta-8 chain, a member of the beta-tubulin protein family that forms heterodimers with alpha-tubulin to build microtubules.
• As a major constituent of microtubules, beta-tubulin participates in dynamic instability through GTP binding and hydrolysis, mediating chromosome segregation, intracellular transport, and cell shape maintenance.
• TUBB8 plays a critical role in meiotic spindle assembly and oocyte maturation, as shown by functional studies. Related references: PMID:26789871 PMID:34509376
• The protein localizes to the cytoplasm, cytoskeleton, and specifically to the meiotic spindle in oocytes (SUBCELLULAR LOCATION).
• Expression is highest in oocytes at various developmental stages, underscoring its importance in female gametogenesis (TISSUE SPECIFICITY).
• Key structural features include GTP-binding and magnesium-binding sites; it is classified as a phosphoprotein with isopeptide bonds (KEYWORDS).
• Disease variants of TUBB8 are associated with oocyte maturation defects (OOMD, OOMD2, OZEMA2), highlighting its essential role in human fertility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 350-444) of human TUBB8, suggesting epitope specificity; consider epitope mapping for confirmation.
• For Western blot (WB), a band around 50 kDa is expected; validate in human oocyte or TUBB8-expressing cell lysates.
• In immunofluorescence (IF/ICC), permeabilization is required to access cytoplasmic and spindle-localized TUBB8; co-staining with spindle markers is recommended.
• For ELISA, the antibody can be used for direct or indirect detection; perform titration to determine optimal working concentrations.
• As a polyclonal antibody, lot-to-lot variability may exist; always validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. Whether you require polyclonal or monoclonal antibodies for diagnostic assay development, bulk quantities, or rare target sourcing, our integrated supply chain supports your project from concept to clinic. For research into microtubule biology and reproductive health, this TUBB8 antibody provides a reliable reagent option, backed by our commitment to quality and rapid delivery.