Anti-OTUB1 Polyclonal Antibody for WB, ELISA - Q96FW1

Core Product Specifications and Parameters

Biological Background: OTUB1 Function and Localization

• OTUB1 is a ubiquitin thioesterase that specifically cleaves 'Lys-48'-linked polyubiquitin chains from proteins, with much lower activity toward NEDD8; it does not cleave di-ubiquitin (PubMed:12401499, 12704427, 14661020, 18954305, 23827681). Related references: PMID:12401499 PMID:12704427 PMID:14661020 PMID:18954305 PMID:23827681
• It regulates T-cell anergy by interacting with RNF128/GRAIL: isoform 1 destabilizes RNF128 to prevent anergy, while isoform 2 stabilizes RNF128 and promotes anergy (PubMed:14661020). Related references: PMID:14661020
• OTUB1 plays a non-catalytic role in DNA repair by inhibiting the E3 ligase RNF168 through binding to UBE2N/UBC13, limiting the spread of 'Lys-63'-linked histone ubiquitination marks at damage sites; this inhibition is allosterically regulated by free ubiquitin (PubMed:20725033, 22325355). Related references: PMID:20725033 PMID:22325355
• It regulates mTORC1 and mTORC2 complexes via non-catalytic deubiquitination of RPTOR (when phosphorylated at Tyr-26) and DEPTOR (PubMed:29382726, 35927303). Related references: PMID:29382726 PMID:35927303
• Deubiquitinates estrogen receptor alpha (ESR1) (PubMed:19383985). Related references: PMID:19383985
• Subcellular location: primarily cytoplasmic.
• Isoform 1 is ubiquitously expressed, whereas isoform 2 is restricted to lymphoid tissues (tonsils, lymph nodes, spleen, peripheral blood mononuclear cells).
• Post-translational modifications include phosphorylation (e.g., Tyr-26) and acetylation, and the protein is implicated in processes such as DNA damage response, adaptive immunity, and thiol protease activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–150 of human OTUB1, a region present in both isoforms; consider this when interpreting results in lymphoid vs. non-lymphoid samples.
• For Western blot (WB), expect a band at approximately 31 kDa. Optimize blocking and antibody dilution for your specific sample type.
• For ELISA, both direct and capture formats may be suitable; validate coating and detection conditions with purified protein or lysates.
• The antibody cross-reacts with mouse OTUB1, so it can be used in mouse models, but verify specificity in your experimental system.
• Consider the functional context: OTUB1 is involved in deubiquitination, DNA repair, and mTOR signaling, so appropriate controls (e.g., ubiquitination status, DNA damage induction) may be needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw materials, offering a one-stop solution from concept to clinic. This anti-OTUB1 antibody supports research into protein turnover, DNA repair, and immune regulation, areas critical for developing novel diagnostics. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk reagents, and custom sourcing for rare targets—streamline your development with CamelBio's integrated supply chain.