Anti-IBSP Rabbit Polyclonal Antibody for WB, ELISA - P21815

Core Product Specifications and Parameters

Biological Background: Integrin-Binding Sialoprotein (IBSP) Function and Localization

• IBSP, also known as bone sialoprotein 2 or BSP II, is a secreted glycoprotein and a member of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family.
• It binds tightly to hydroxyapatite and forms an integral part of the mineralized bone matrix, contributing to biomineralization. Related references: PMID:11459848, PMID:1818768
• IBSP promotes cell adhesion and migration through interaction with the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3), facilitating cell-matrix communication. Related references: PMID:10640428, PMID:11459848, PMID:24103036
• The protein is primarily expressed in bone tissue, where it is a major non-collagenous component of the extracellular matrix. Related references: PMID:11459848
• It is also expressed in trophoblast cells of the placenta and has been detected in brain tissue. Related references: PMID:1818768, PMID:36261010
• IBSP undergoes post-translational modifications including phosphorylation, sulfation, and sialic acid addition, which may influence its function.
• The protein contains a signal peptide for secretion and has a calculated molecular weight of approximately 35 kDa.

Experimental Guidance and Technical Tips

• Given that the immunogen is a synthetic peptide from the N‑terminal region (amino acids 1–100), the antibody may detect full‑length IBSP as well as potential processed isoforms; confirm specificity in your assay system.
• For Western blotting, a band near 35 kDa is expected, but migration may be shifted due to extensive post‑translational modifications; consider using reducing conditions and appropriate molecular weight markers.
• In ELISA applications, the antibody can be used for capture or detection; titrate and validate using relevant recombinant protein or biological samples.
• Cross‑reactivity with mouse and rat IBSP is reported; however, sequence alignment and pilot testing in the target species are recommended to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop partner for IVD raw materials, offering validated polyclonal antibodies like anti‑IBSP for bone metabolism and cell adhesion research, alongside monoclonal antibody pairs for immunoassay development. We also provide bulk ancillary reagents and rare‑target sourcing to streamline your entire diagnostic workflow from concept to clinic.