Anti-RPS7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62081

Core Product Specifications and Parameters

Biological Background: RPS7 Function and Localization

• RPS7 is a core component of the small (40S) ribosomal subunit, essential for cellular protein synthesis. Related references: PMID:23636399
• It is required for the maturation of ribosomal RNA (rRNA). Related references: PMID:19061985
• RPS7 is integral to the small subunit (SSU) processome, the earliest precursor of the small eukaryotic ribosomal subunit, coordinating pre-rRNA folding, modification, and cleavage. Related references: PMID:34516797
• The protein localizes to the cytoplasm, centrosome, nucleolus, and nucleus, reflecting its roles in ribosome biogenesis and function.
• Post-translational modifications include acetylation, isopeptide bond formation, and ubiquitin conjugation (Ubl).
• Mutations in RPS7 are linked to Diamond-Blackfan anemia, a ribosomopathy.

Experimental Guidance and Technical Tips

• The immunogen, comprising residues 1-194 of human RPS7, targets the near full-length protein; consider including a positive control lysate from human, mouse, or rat cells.
• For Western blot, a band at ~22 kDa is expected under reducing conditions; validate using appropriate molecular weight markers.
• For IHC-P and IF/ICC, optimize antigen retrieval and primary antibody dilution in the relevant tissue or cell system.
• ELISA may be used for quantitative detection of RPS7; include appropriate standards and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your ribosomal protein research with a comprehensive portfolio of ready-to-use antibodies, including this anti-RPS7 polyclonal antibody, ensuring reliable detection across multiple applications. As a one-stop IVD raw material sourcing partner for diagnostic manufacturers and research labs, we supply optimized antibody pairs, bulk ancillary reagents, and can source rare targets for ribosome biogenesis and ribosomopathy studies. Accelerate your diagnostic assay development with our integrated supply and consulting services.