Anti-IL36R Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9HB29

Core Product Specifications and Parameters

Biological Background: IL36R Function and Localization

• Interleukin-1 receptor-like 2 (IL36R), also known as IL-1Rrp2 or IL-1 receptor-related protein 2, is a receptor for the interleukin-36 cytokines (IL36A, IL36B, and IL36G) and belongs to the interleukin-1 receptor family.
• Upon ligand binding, IL36R associates with the coreceptor IL1RAP to form a functional receptor complex, which triggers downstream signaling cascades including NF-kappa-B and MAPK pathways (By similarity).
• The IL-36 signaling system is localized to epithelial barriers and plays a role in local inflammatory responses, analogous to the IL-1 system, and is particularly implicated in skin inflammation through the induction of the IL-23/IL-17/IL-22 pathway.
• IL36R is a membrane protein with a calculated molecular weight of approximately 65.4 kDa, and it undergoes post-translational modifications including glycosylation and disulfide bond formation.
• Tissue expression of IL36R is observed in synovial fibroblasts, articular chondrocytes, keratinocytes, monocyte-derived dendritic cells, monocytes, and myeloid dendritic cells, as confirmed at the protein level.
• The receptor features extracellular immunoglobulin-like domains and a cytoplasmic TIR domain, characteristic of the interleukin-1 receptor family.
• Alternative splicing of the IL1RL2 gene leads to multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-116), which may recognize both full-length and extracellular domains of IL36R. Consider this when designing detection assays for soluble receptor forms or membrane-bound receptor.
• For Western blot (WB), the predicted molecular weight is 65 kDa; however, glycosylation may cause the band to appear higher. Validate in relevant cell lysates known to express IL36R (e.g., keratinocytes, monocytes).
• For immunofluorescence/immunocytochemistry (IF/ICC), membrane localization is expected. Include appropriate permeabilization controls to distinguish surface staining from intracellular pools.
• Cross-reactivity with mouse IL36R is reported, making this antibody suitable for studies in mouse models. However, always validate specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including optimized antibody pairs and bulk reagents. For your IL36R-related inflammatory and dermatological research, we supply high-quality polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents to accelerate assay development. Through our integrated sourcing platform, we bridge the gap from concept to clinic, ensuring reliable and consistent supply for your diagnostic workflows.