Anti-RAB3IP Polyclonal Antibody for WB, ELISA - Q96QF0

Core Product Specifications and Parameters

Biological Background: RAB3IP Function and Localization

• RAB3IP (Rab-3A-interacting protein), also known as Rabin-3, Rabin8, and SSX2-interacting protein, is a guanine nucleotide exchange factor (GEF) that specifically activates RAB8A and RAB8B, promoting the exchange of GDP for GTP to convert them into their active GTP-bound forms. Related references: PMID:12221131, PMID:26824392

• RAB3IP mediates the release of GDP from RAB8A and RAB8B, but does not act on RAB3A or RAB5, indicating specificity within the Rab family. Related references: PMID:20937701, PMID:26824392

• It modulates actin organization and facilitates polarized transport of RAB8A-specific vesicles to the cell surface, contributing to intracellular trafficking and membrane dynamics. Related references: PMID:12221131

• In conjunction with RAB11A, RAB8A, the exocyst complex, and other proteins, RAB3IP promotes transcytosis of PODXL to apical membrane initiation sites, aiding in apical surface formation and lumenogenesis. Related references: PMID:20890297

• As a component of a ciliary targeting complex with Rab11, ASAP1, RAB11FIP3, and ARF4, RAB3IP drives preciliary vesicle trafficking to the mother centriole and is essential for ciliogenesis initiation. Related references: PMID:25673879, PMID:31204173

• The protein localizes to the cytoplasm, nucleus, cytoskeleton, lamellipodium, vesicles, and centrosome, reflecting its versatile roles in cellular trafficking and organization.

• Tissue expression analysis reveals RAB3IP in brain, kidney, heart, pancreas, and placenta, with no detectable expression in skeletal muscle or liver.

• RAB3IP is annotated with UniProt keywords including Alternative splicing, Coiled coil, Guanine-nucleotide releasing factor, Phosphoprotein, and Protein transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-150) of human RAB3IP, which may influence epitope recognition; validate the antibody's reactivity with your target sequence if modifications or truncations are expected.

• For Western blot, a band around 53 kDa (predicted molecular weight) should be observed; use appropriate protein extraction and loading controls to confirm specificity.

• Cross-reactivity data with mouse and rat samples suggest suitability for these species; however, verify compatibility in your specific tissue or cell line.

• ELISA applications may require optimization of coating antigen, antibody concentration, and detection system; consider pairing with a validated secondary antibody.

• As a polyclonal antibody, note potential lot-to-lot variability; conduct pilot experiments when switching lots to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including antibodies such as this RAB3IP pAb, to support research in vesicle trafficking and ciliogenesis. Our supply capabilities span validated antibody pairs, bulk ancillary reagents, and customized rare-target raw-material procurement, helping diagnostic manufacturers and labs streamline assay development from concept to clinic.