Anti-IL8 Rabbit Polyclonal Antibody for WB, ELISA - P10145

Core Product Specifications and Parameters

Biological Background: Interleukin-8 Function and Localization

• Interleukin-8 (IL-8), also known as C-X-C motif chemokine 8 (CXCL8), is a secreted chemokine and member of the CXC chemokine family, encoded by the CXCL8 gene.
• IL-8 acts as a chemotactic factor, mediating inflammatory responses by attracting neutrophils, basophils, and T-cells to sites of infection, thereby contributing to pathogen clearance and host defense (PubMed:18692776, PubMed:7636208). Related references: PMID:18692776 PMID:7636208
• It also plays a crucial role in neutrophil activation, a key event in the innate immune response (PubMed:2145175, PubMed:9623510). Related references: PMID:2145175 PMID:9623510
• Upon release, IL-8 exerts its effects by binding to the G protein-coupled receptors CXCR1 and CXCR2, which are primarily expressed on neutrophils, monocytes, and endothelial cells (PubMed:1840701, PubMed:1891716). Related references: PMID:1840701 PMID:1891716
• Ligand binding triggers dissociation of the G protein heterotrimer, leading to release of beta and gamma subunits from Galpha (GNAI2 in neutrophils) and activation of downstream signaling cascades, including the PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698). Related references: PMID:11971003 PMID:8662698
• IL-8 is a secreted protein, and its post-translational modifications include citrullination and the formation of disulfide bonds, which are essential for its stability and function.
• The mature protein has a molecular weight of approximately 11 kDa (11098 Da) and is a key player in acute and chronic inflammatory diseases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature peptide region (aa 28-99), which includes the receptor-binding domain; this design may help detect native IL-8 in biological samples, but cross-reactivity with other CXC chemokines should be assessed.
• For Western blotting, IL-8 contains disulfide bonds; reducing and denaturing conditions may be required for consistent detection of the ~11 kDa band.
• In ELISA applications, consider pairing this polyclonal antibody with a suitable capture or detection antibody; optimization of coating and detection conditions is recommended.
• Since IL-8 is secreted, cell culture supernatants or serum samples may require appropriate collection and stabilization protocols to prevent degradation.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material partner, CamelBio supports diagnostic manufacturers and research institutes with one-stop access to validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-IL8 polyclonal antibody is suitable for chemokine research and immunoassay development, and our team can assist with sourcing rare targets and custom raw material solutions to streamline your workflow from concept to clinic.