IL4 Rabbit Polyclonal Antibody for WB, ELISA - P05112

Core Product Specifications and Parameters

Biological Background: Interleukin-4 Function and Localization

• Interleukin-4 (IL-4) is a secreted cytokine, also known as BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, and Pitrakinra.
• Produced by mast cells, T-cells, eosinophils, and basophils, IL-4 is a central regulator of humoral and adaptive immunity, including antibody class switching and effector T-cell responses.
  Related references: PMID:1993171, PMID:3016727
• IL-4 induces class II MHC expression on resting B-cells and enhances secretion and cell surface expression of IgE and IgG1.
  Related references: PMID:1993171
• It also regulates expression of the low-affinity IgE receptor (CD23) on lymphocytes and monocytes.
  Related references: PMID:2521231
• IL-4 positively regulates IL31RA in macrophages and stimulates autophagy in dendritic cells via mTORC1 inhibition and RUFY4 induction.
• Signaling occurs via two receptor complexes: type 1 (IL4R/IL2RG) on hematopoietic cells and type 2 (IL4R/IL13RA1) on non-hematopoietic cells, leading to JAK/STAT6 pathway activation.
  Related references: PMID:10219247, PMID:11526337, PMID:18243101, PMID:7721895
• Beyond immune functions, IL-4 plays a role in normal brain physiology, including memory and learning (By similarity).
• The protein is secreted, glycosylated, and contains disulfide bonds; its molecular weight is approximately 17.5 kDa, with multiple isoforms from alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide from the N-terminal region (aa 1-100) of human IL-4; this sequence is largely conserved in mouse and rat, supporting the reported cross-reactivity.
• For Western blot, consider using reducing conditions and include appropriate positive controls such as stimulated T-cell or mast cell lysates. The predicted band is approximately 17 kDa, but glycosylation may cause slight shifts.
• In ELISA applications, the antibody can be explored as a capture or detection reagent; pairing with a matched monoclonal may improve sensitivity. Confirm performance in your specific sample matrix.
• When testing cross-reactivity in mouse or rat samples, validate with known positive and negative controls due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For immune-related targets like IL-4, we offer validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to accelerate your immunoassay development. Backed by robust supply chains and technical support, we streamline your sourcing workflow, ensuring reliable material supply for IVD applications.