Anti-IL23R Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q5VWK5

Core Product Specifications and Parameters

Biological Background: Interleukin-23 Receptor Function and Localization

• The interleukin-23 receptor (IL23R) is a type I cytokine receptor that pairs with IL12RB1 to form a heterodimeric receptor; it binds IL23, initiating pro-inflammatory signaling.
• IL23R signaling activates the Jak-Stat pathway and plays a pivotal role in innate and adaptive immunity, promoting the differentiation and maintenance of Th17 cells and stimulating NK cells and certain myeloid populations.
• Subcellularly, IL23R localizes to the cell membrane, where it mediates extracellular ligand recognition and intracellular signal transduction.
• IL23R is alternatively spliced, yielding at least two isoforms; isoform 1 shows enriched expression in NK cells, while the receptor is broadly detected on monocytes, Th1/Th0 cells, and dendritic cells.
• Post-translational modifications include N-linked glycosylation and phosphorylation, which are critical for receptor maturation, ligand binding, and downstream signaling.
• Given its role in driving chronic inflammation, IL23R has been implicated in autoimmune diseases such as psoriasis, inflammatory bowel disease, and ankylosing spondylitis, and may also contribute to tumorigenesis.
• The molecular weight of the mature protein is ~72 kDa (calculated from sequence), consistent with the observed band on Western blot.

Experimental Guidance and Technical Tips

• For Western blot (WB), the antibody detects a band around 72 kDa in human, mouse, and rat samples; validate in the relevant species with appropriate controls.
• For immunofluorescence/ICC and immunohistochemistry, expect membrane staining with possible intracellular pools; include permeabilization if targeting the cytoplasmic epitope.
• For ELISA applications, the polyclonal nature may enhance sensitivity; optimize as capture or detection reagent depending on the format.
• The immunogen corresponds to the C-terminal region (residues 510–629), so the antibody likely recognizes the intracellular domain; this is advantageous for detecting total receptor levels independent of extracellular domain shedding.
• Cross-reactivity with mouse and rat IL23R has been confirmed; verify sequence homology in the immunogen region to ensure comparable binding.
• As a rabbit polyclonal antibody, it may detect multiple isoforms; confirm specificity by siRNA knockdown or overexpression controls.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio supports your diagnostic development with this high-quality anti-IL23R polyclonal antibody. From validated antibody pairs and optimized monoclonal/polyclonal preparations to bulk ancillary reagents and rare target sourcing, CamelBio bridges the gap between innovative biomarker research and clinical assay manufacturing. Whether investigating IL23/Th17-driven autoimmunity or developing IL23R-based diagnostics, our comprehensive portfolio streamlines your raw-material supply chain.