Anti-IRF8 Rabbit Polyclonal Antibody for WB and ELISA - Q02556

Core Product Specifications and Parameters

Biological Background: IRF8 Function and Localization

• IRF8, also known as interferon consensus sequence-binding protein (ICSBP), is a member of the interferon regulatory factor (IRF) family of transcription factors.
• It specifically binds to the interferon consensus sequence (ICS) upstream of type I IFN and IFN-inducible MHC class I genes, acting as both a transcriptional activator and repressor.
  Related references:
  PMID:25122610
• IRF8 plays a critical role in immune cell development, including the differentiation of CD8+ dendritic cells through complex formation with BATF-JUNB and recognition of AICE sequences, as well as the development of plasmacytoid dendritic cells (pDCs) that produce type I IFN.
• It positively regulates macroautophagy in dendritic cells.
  Related references:
  PMID:29434592
• IRF8 also acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1.
• The protein contains key functional domains for DNA binding and transcriptional regulation, and its activity is modulated by ubiquitin-like conjugation.
• Subcellularly, IRF8 is found in both the nucleus and the cytoplasm, consistent with its role as a transcription factor that shuttles between compartments.
• It is predominantly expressed in lymphoid tissues, reflecting its immune-specific functions.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment spanning amino acids 137–426 of human IRF8, covering the majority of the protein including its DNA-binding and regulatory regions. Consider this broad immunogen coverage when interpreting multi-band patterns in WB.
• For Western blot detection of IRF8, whole cell lysates from human or mouse immune cells such as peripheral blood mononuclear cells (PBMCs) or dendritic cell lines are appropriate sample types. Nuclear enrichment may improve sensitivity given IRF8’s role as a transcription factor.
• In ELISA applications, this antibody may be used for capture or detection in sandwich immunoassays. Validate pairing with a suitable monoclonal antibody or use recombinant IRF8 protein as a standard for quantification.
• Cross-reactivity with mouse IRF8 is confirmed; however, always validate performance in your specific sample and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop supply chain for IVD raw materials. This anti-IRF8 polyclonal antibody exemplifies our portfolio for immunology and transcription factor research, supported by our global sourcing network that provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target raw materials. Let us accelerate your assay development from concept to clinic with reliable, research-grade reagents.