Anti-RPLP0 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05388

Core Product Specifications and Parameters

Biological Background: RPLP0 Function and Localization

• RPLP0, also known as 60S acidic ribosomal protein P0 or 60S ribosomal protein L10E, is a key component of the large ribosomal subunit.
• It is the functional equivalent of E. coli ribosomal protein L10, essential for ribosome assembly and function.
• The protein is encoded by the RPLP0 gene (Gene ID: 6175) in humans.
• RPLP0 localizes to both the nucleus and cytoplasm, consistent with its role in ribosome biogenesis and protein synthesis.
• It is an acidic phosphoprotein, as indicated by its alternative name, suggesting regulation by phosphorylation.
• The protein undergoes post-translational modifications including phosphorylation and ubiquitination (Isopeptide bond, Ubl conjugation), which may modulate its activity.
• RPLP0 has a molecular weight of approximately 34 kDa and is conserved across species, showing cross-reactivity with mouse.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (amino acids 1-317). For Western blot, consider using whole-cell lysates and standard denaturing conditions to detect the ~34 kDa band.
• In immunofluorescence or ICC, anticipate nuclear and cytoplasmic staining; include appropriate controls to validate specificity.
• For ELISA applications, the antibody may be used with recombinant RPLP0 protein or cell extracts; consider optimizing coating and detection conditions.
• Cross-reactivity with mouse RPLP0 is indicated; validate in the relevant sample system if using mouse models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support every stage of diagnostic development. For ribosomal protein research, including RPLP0 and related targets, we provide reliable reagents and custom sourcing solutions. Let CamelBio streamline your supply chain from concept to clinic.