Anti-ACC1 Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q13085

Core Product Specifications and Parameters

Biological Background: ACC1 Function and Localization

• ACC1 (acetyl-CoA carboxylase 1) is a cytosolic enzyme that catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. Related references: PMID:20457939, PMID:20952656, PMID:29899443
• This biotin-dependent multifunctional enzyme contains distinct domains for biotin carboxylation, carboxyl transfer, and biotin carrier activity, highlighting its complex regulatory role in lipid metabolism.
• ACC1 is an allosteric enzyme regulated by phosphorylation and acetylation, which modulate its activity in response to metabolic signals.
• It resides primarily in the cytoplasm, where it provides malonyl-CoA for long-chain fatty acid synthesis.
• Tissue expression is widespread, including brain, placenta, skeletal muscle, kidney, pancreas, and adipose tissues, but is absent in liver, suggesting tissue-specific metabolic functions.
• Alternative names include acetyl-CoA carboxylase alpha (ACC-alpha), reflecting its distinction from the mitochondrial ACC2 isoform involved in fatty acid oxidation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a highly conserved region (amino acids 350–450) of human ACC1; cross-reactivity with mouse and rat is supported, but confirm specificity via peptide competition or genetic controls.
• For Western blotting, ACC1 has a high molecular weight (approximately 266 kDa); consider using a gradient gel (e.g., 4–12%) and extended wet transfer (e.g., overnight at 30 V) to ensure efficient transfer.
• In IHC-P, conduct antigen retrieval (e.g., citrate buffer, pH 6) and validate with a known positive tissue such as human placenta or adipose; include a no-primary control.
• For IP, pilot small-scale experiments to determine optimal antibody-to-lysate ratios and bead conjugation conditions.
• ELISA applications may benefit from pairing with a matched capture antibody or direct labeling; perform checkerboard titrations for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents for metabolic disease research. This anti-ACC1 rabbit monoclonal antibody exemplifies our commitment to providing high-quality reagents for fatty acid metabolism studies, with reliable supply chains and technical support to accelerate diagnostic assay development. Whether you need bulk antibody production or customized rare-target sourcing, CamelBio streamlines your path from concept to clinic.