Anti-AKT3 Monoclonal Antibody for WB, ELISA - Q9Y243

Core Product Specifications and Parameters

Biological Background: AKT3 Function and Localization

• RAC-gamma serine/threonine-protein kinase (AKT3) is one of three closely related AKT kinase isoforms (AKT1, AKT2, AKT3) that regulate metabolism, proliferation, cell survival, growth, and angiogenesis through phosphorylation of downstream substrates.
• Over 100 substrate candidates have been reported, but isoform specificity remains largely uncharacterized; AKT3 is the least studied AKT isoform.
• AKT3 plays a critical role in brain development and is essential for the viability of malignant glioma cells; it may also regulate MMP13 expression via IL13 signaling.
• AKT3 is required for coordinating mitochondrial biogenesis with growth factor-induced increases in cellular energy demands; its downregulation reduces phosphorylated BAD, inducing caspase-dependent apoptosis.
• Subcellular localization includes the nucleus, cytoplasm, and membrane.
• In adult tissues, AKT3 is highly expressed in brain, lung, and kidney, but weakly in heart, testis, and liver; in fetal tissues, it is highly expressed in heart, liver, and brain, but absent in kidney.
• Keywords associated with AKT3 include kinase, transferase, phosphoprotein, nucleotide-binding, ATP-binding, and disease variant, reflecting its central role in cellular signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 93–154 of human AKT3; consider epitope accessibility and conservation when using in non-human species.
• For WB analysis, a band around 56 kDa is expected; validate performance using lysates from brain, lung, or kidney tissues where AKT3 is highly expressed.
• This antibody has been applied in ELISA; appropriate blocking and detection conditions should be optimized in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For AKT3-related projects in cancer, metabolism, or neuroscience, we streamline sourcing from concept to clinic. Our platform also supports rare target raw-material procurement, ensuring your development stays on track.