Anti-ACVR1B Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P36896

Core Product Specifications and Parameters

Biological Background: ACVR1B Function and Localization

• ACVR1B, also known as Activin receptor type-1B, ALK4, SKR2, and ACTRIB, is a transmembrane serine/threonine kinase receptor that mediates signaling by activins, members of the TGF-beta superfamily.
• It forms a heteromeric receptor complex with type-2 activin receptors (ACVR2A or ACVR2B) at the cell membrane; ligand binding to the type-2 receptor leads to phosphorylation and activation of ACVR1B.
• Upon activation, ACVR1B directly phosphorylates the downstream transcription factors SMAD2 and SMAD3 on their C-terminal serine residues, which then partner with SMAD4 to translocate into the nucleus and regulate gene expression.
• ACVR1B signaling is implicated in numerous physiological and pathological processes, including neuronal differentiation, hair follicle development and cycling, FSH production, wound healing, extracellular matrix production, immunosuppression, and carcinogenesis.
• Subcellularly, ACVR1B is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue expression is broad, with highest levels reported in kidney, pancreas, brain, lung, and liver.
• According to UniProt, ACVR1B is subject to phosphorylation, glycosylation, and alternative splicing, and it features ATP-binding, nucleotide-binding, and metal-binding domains required for its kinase activity.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide corresponding to amino acids 1-100 of human ACVR1B (P36896), which covers the N-terminal signal sequence and part of the extracellular domain. This region is predicted to be accessible and may be suitable for detecting both full-length and processed receptor forms.
• For western blotting, a specific band around 57 kDa is expected under reducing conditions, consistent with the predicted molecular weight of ACVR1B.
• In immunohistochemistry (IHC-P), researchers may consider using tissues with high ACVR1B expression, such as kidney or liver, as positive controls; appropriate antigen retrieval and antibody titration are recommended.
• For immunofluorescence (IF/ICC), membrane localization should be observed; co-staining with compartment-specific markers can confirm specificity.
• ELISA assays can be developed using this antibody ; for quantitative detection, pairing with a validated capture antibody is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, supporting diagnostic manufacturers and research laboratories from early development to clinical application. This anti-ACVR1B monoclonal antibody is part of our portfolio for activin and TGF-beta signaling research, available alongside validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need optimized clones or scalable raw materials, CamelBio serves as your reliable supply partner.