Anti-ACAT1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P24752

Core Product Specifications and Parameters

Biological Background: ACAT1 Function and Localization

• ACAT1 encodes mitochondrial acetyl-CoA acetyltransferase, also known as acetoacetyl-CoA thiolase (T2), a crucial enzyme in fatty acid beta-oxidation.
  Related references: PMID:1715688, PMID:7728148, PMID:9744475
• It catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a shortened acyl-CoA, using free coenzyme A, which is the final step of beta-oxidation.
  Related references: PMID:1715688, PMID:7728148, PMID:9744475
• The enzyme operates reversibly and can condense two acetyl-CoA molecules to form acetoacetyl-CoA, thus playing a key role in ketone body metabolism.
  Related references: PMID:17371050, PMID:1715688, PMID:7728148
• ACAT1 is localized to the mitochondrial matrix.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing generating multiple isoforms.
• Mutations in ACAT1 are linked to disease variants, emphasizing its importance in metabolic homeostasis.
• Keywords associated with ACAT1 include 3D-structure, metal-binding (potassium), proteomics identification, and reference proteome.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band around 45 kDa is expected. Use appropriate positive controls expressing ACAT1 (e.g., liver or kidney mitochondria extracts).
• For immunohistochemistry (IHC-P), antigen retrieval protocols should be optimized. Mitochondrial staining pattern is anticipated.
• For immunofluorescence (IF/ICC), confirm mitochondrial localization by co-staining with a mitochondrial marker.
• For ELISA, validate linearity and specificity using recombinant ACAT1 protein or target-expressing cell lysates.
• When extending to mouse or rat samples, note that cross-reactivity is reported, but validation in the relevant species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive sourcing platform for IVD raw materials. For metabolic and mitochondrial research, including studies on ACAT1 and fatty acid oxidation, we offer validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. Our expertise in rare-target sourcing ensures timely access to critical components for assay development.