Anti-ACAT1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P24752

Core Product Specifications and Parameters

Biological Background: ACAT1 Function and Localization

• Acetyl-CoA acetyltransferase, mitochondrial, also known as acetoacetyl-CoA thiolase (T2), is encoded by the ACAT1 gene.
• Catalyzes the last step of mitochondrial fatty acid beta-oxidation: thiolytic cleavage of 3-oxoacyl-CoAs into acetyl-CoA and a shortened acyl-CoA. Reversibly catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA, playing a major role in ketone body metabolism. Related references: PMID:1715688, PMID:7728148, PMID:9744475, PMID:17371050
• Localized to the mitochondrion, consistent with its central role in fatty acid oxidation and ketogenesis.
• Belongs to the thiolase family of enzymes, which are critical for lipid metabolism.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing.
• Contains a potassium ion binding site, as indicated by metal-binding properties.
• Mutations in ACAT1 are associated with metabolic disorders, as noted in disease variant annotations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 328–427 of human ACAT1, suggesting the antibody recognizes a linear epitope in the C-terminal region.
• In Western blot (WB), a specific band is expected at approximately 45 kDa. Consider using reducing conditions and validate with appropriate positive controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; citrate-based methods are commonly used. Optimize antibody dilution and detection conditions.
• ELISA performance may depend on coating antigen and detection format; titration experiments are recommended.
• The antibody cross-reacts with rat ACAT1; validate in the relevant species and tissue systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs seamless access to premium IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-ACAT1 clone. Our supply capabilities extend to bulk ancillary reagents and rare target sourcing, supporting projects from discovery to clinical application. For lipid metabolism and metabolic disorder research, we provide integrated procurement solutions to streamline your workflow.