Anti-ACC1 (KO Validated) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5SWU9

Core Product Specifications and Parameters

Biological Background: ACC1 (Acetyl-CoA carboxylase 1) Function and Localization

• ACC1, also known as ACC-alpha or Acetyl-CoA carboxylase 265, is a 265 kDa biotin-dependent enzyme encoded by the Acaca gene in mouse.
• It catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step in de novo fatty acid biosynthesis. Related references: PMID:20952656
• The reaction proceeds in two steps: ATP-dependent carboxylation of the biotin prosthetic group, followed by transfer of the carboxyl group to acetyl-CoA.
• ACC1 is a multidomain protein containing biotin carboxylase, biotin carboxyl carrier, and carboxyltransferase domains, enabling its multifunctional enzyme activity.
• Subcellular localization is predominantly cytosolic, consistent with its role in cytoplasmic fatty acid synthesis.
• Post-translational modifications such as acetylation and phosphorylation regulate ACC1 activity in response to metabolic cues.
• The enzyme requires divalent cations like magnesium or manganese for its catalytic function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a human ACC1 sequence (aa 2246–2345); expected cross-reactivity with human, mouse, and rat should be validated in your specific sample system.
• For Western blotting, ACC1 is a large protein (~265–270 kDa); use appropriate gel percentage and transfer conditions to ensure efficient detection.
• For IHC-P, optimize antigen retrieval and antibody dilution empirically; tissues with high lipogenic activity (e.g., liver, adipose) may serve as positive controls.
• As a polyclonal antibody, batch-to-batch consistency should be monitored; test new lots before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for diagnostic manufacturers and research laboratories, offering a complete range of IVD raw materials from validated antibodies to bulk ancillary reagents. This ACC1 polyclonal antibody supports investigations into fatty acid metabolism, metabolic disorders, and obesity-related research, and CamelBio's sourcing capabilities can extend to custom antibody development and rare target raw-material procurement to accelerate your project from concept to clinic.