Anti-AKT1/2 Rabbit Monoclonal Antibody for WB, IHC, ELISA - P31749 / P31751

Core Product Specifications and Parameters

Biological Background: AKT1/2 Function and Localization

• AKT1 is a serine/threonine-protein kinase belonging to the AKT family, which includes AKT2 and AKT3. It acts as a key regulator of metabolism, proliferation, cell survival, growth, and angiogenesis (PubMed:11882383, PubMed:15526160, PubMed:15861136). Related references: PMID:11882383, PMID:15526160, PMID:15861136
• It phosphorylates a broad range of downstream substrates, with over 100 candidates identified, although isoform specificity is not well-defined for most (PubMed:11882383, PubMed:15526160, PubMed:21432781). Related references: PMID:11882383, PMID:15526160, PMID:21432781
• In insulin signaling, AKT1 promotes glucose uptake by mediating translocation of the GLUT4 transporter to the cell surface and regulates glycogen synthesis by inhibiting GSK3α and GSK3β (By similarity).
• AKT1 promotes cell survival by phosphorylating and inhibiting pro-apoptotic factors: MAP3K5 at Ser-83 (PubMed:11154276), BAD (PubMed:10926925), and FOXO transcription factors, leading to their cytoplasmic sequestration (PubMed:10358075). Related references: PMID:11154276, PMID:10926925, PMID:10358075
• It is a central component of the PI3K/AKT/mTOR pathway, activating mTORC1 via TSC2 phosphorylation (PubMed:12150915, PubMed:12172553) and modulating mTORC2 activity through MAPKAP1/SIN1 phosphorylation (By similarity). Related references: PMID:12150915, PMID:12172553
• AKT1 localizes to the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, reflecting its involvement in diverse signaling compartments.
• It is ubiquitously expressed in human cells, with elevated expression and activation observed in cancers such as prostate and breast, where Tyr-176 phosphorylation increases during progression.
• Post-translational modifications, including phosphorylation, acetylation, ubiquitylation, and glycosylation, tightly regulate AKT1 activity, stability, and interactions.

Experimental Guidance and Technical Tips

• The immunogen peptide spans amino acids 381-480 of human AKT1 (P31749), a region predicted to be conserved in AKT2; thus, the antibody may recognize both AKT1 and AKT2. Consider verifying isoform specificity in your experimental system.
• For Western blot (WB), the antibody detects a band around 56 kDa. Validate the molecular weight using positive control lysates (e.g., HeLa, NIH/3T3) and appropriate loading controls.
• In immunohistochemistry (IHC-P), ensure proper antigen retrieval and validate staining patterns using tissue sections known to express AKT1/2, such as prostate or breast cancer tissues.
• For ELISA, consider pairing with a detection antibody targeting a different epitope for sandwich ELISA development. Optimize coating and blocking conditions.
• Since AKT1/2 are phosphorylated upon activation, the antibody may detect total AKT; for activation-specific studies, pair with phospho-specific antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering validated antibody pairs, bulk ancillary reagents, and customized monoclonal/polyclonal antibody development. This AKT1/2 rabbit monoclonal antibody supports research into AKT signaling in cancer and metabolism. We also assist with rare target raw material procurement to accelerate your assay development from concept to clinic.