AKT1 Rabbit Monoclonal Antibody (KO Validated) for WB, IHC-P, IF/ICC, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 (RAC-alpha serine/threonine-protein kinase, PKB alpha) is a key serine/threonine kinase in the AKT family, regulating diverse cellular processes including metabolism, proliferation, survival, and angiogenesis.
  Related references: PMID:11882383, PMID:15526160, PMID:15861136, PMID:21432781

• AKT1 localizes to multiple subcellular compartments: cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, enabling context-specific signaling.

• AKT1 is ubiquitously expressed, with significantly elevated protein levels in prostate cancer and increased activating phosphorylation in progressive breast cancer stages.

• AKT1 is a phosphoprotein subject to post-translational modifications including acetylation, glycosylation, and ubiquitin-like conjugation, which regulate its activity, localization, and stability.

• In insulin-responsive tissues, AKT1 promotes glucose uptake by phosphorylating TBC1D4 (AS160), triggering GLUT4 translocation.
  Related references: PMID:11994271

• AKT1 suppresses apoptosis by directly phosphorylating and inhibiting pro-apoptotic factors such as MAP3K5/ASK1 and BAD.
  Related references: PMID:11154276, PMID:10926925

• AKT1 activates mTORC1 signaling by phosphorylating TSC2, promoting protein synthesis and cell growth.
  Related references: PMID:12150915, PMID:12172553

• AKT1 regulates transcription by phosphorylating FOXO factors (FOXO1, FOXO3, FOXO4), leading to their cytoplasmic retention and attenuation of pro-apoptotic and cell cycle arrest programs.
  Related references: PMID:10358075

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 380-480 of human AKT1. Consider using a blocking peptide or recombinant AKT1 protein to verify antibody binding specificity.
• This antibody has been reported to detect AKT1 in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC-P), Immunofluorescence/Immunocytochemistry (IF/ICC), and ELISA. A band of approximately 56 kDa is expected in WB.
• Cross-reactivity with mouse and rat is indicated; validate the antibody's performance in these species with appropriate positive and negative controls.
• As a KO-validated monoclonal antibody, it offers high target specificity; confirm knockout efficiency in your cell lines to ensure reliable results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials, including this high-quality AKT1 rabbit monoclonal antibody. Our comprehensive portfolio features validated antibody pairs, custom monoclonal and polyclonal antibody development, and bulk reagent supply to support your AKT signaling studies and assay development. From concept to clinic, CamelBio is your dependable partner for rare and standard IVD raw materials.