Anti-MMP1 Rabbit Monoclonal Antibody for WB, ELISA - P03956

Core Product Specifications and Parameters

Biological Background: MMP1 Function and Localization

• Interstitial collagenase (MMP-1), also known as fibroblast collagenase, is a member of the matrix metalloproteinase (MMP) family that degrades extracellular matrix components.
• Cleaves fibrillar collagens of types I, II, and III at a single site in the helical domain, as well as non-fibrillar collagens types VII and X, playing a key role in tissue remodeling. Related references: PMID:1645757, PMID:2153297, PMID:2557822
• In the context of HIV infection, MMP1 interacts with and cleaves the secreted viral Tat protein, which can decrease Tat-mediated neurotoxicity. Related references: PMID:16807369
• Localized to the extracellular space and extracellular matrix after secretion, where it exerts its proteolytic activity.
• Contains a signal peptide, an autoinhibitory propeptide with a cysteine switch, a catalytic domain with a zinc-binding motif, and hemopexin-like repeats; activated by autocatalytic cleavage.
• Requires calcium and zinc as cofactors for structural stability and catalytic activity, respectively.
• Annotated with keywords including collagen degradation, host-virus interaction, zymogen, phosphoprotein, and glycoprotein, reflecting its multifaceted regulation and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 93–210 of human MMP1, which covers the C-terminal part of the propeptide and the N-terminal region of the catalytic domain; the antibody may recognize both pro and active forms.
• In western blotting, a band around 54 kDa is expected for the full-length proenzyme; additional lower molecular weight bands corresponding to activated forms may appear depending on sample processing and autocatalysis.
• For ELISA, note that MMP1 can be present in latent or active forms; consider assay format and sample handling conditions to ensure optimal detection.
• The antibody cross-reacts with mouse and rat MMP1, enabling its use in rodent models; validate reactivity in your specific tissue or cell line.
• This reagent is suitable for research applications and IVD raw material use; optimize working dilutions for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials. Our MMP1 rabbit monoclonal antibody offers high specificity for collagenase studies and is part of our growing collection of extracellular matrix-targeting antibodies. As your one-stop bridge, CamelBio supports your entire development workflow with validated antibody pairs, optimized bulk reagents, and sourcing for specialized targets.